Magnetic bead technology for viral RNA extraction from serum in blood bank screening

Show simple item record Albertoni, Guilherme Ambrozio [UNIFESP] Arnoni, Carine Prisco Araujo, Patricia Regina Barboza Andrade, Sheila Siqueira [UNIFESP] Carvalho, Fabrício Oliveira Girão, Manoel João Batista Castello [UNIFESP] Schor, Nestor [UNIFESP] Barreto, José Augusto 2015-06-14T13:43:20Z 2015-06-14T13:43:20Z 2011-12-01
dc.identifier.citation Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 6, p. 547-552, 2011.
dc.identifier.issn 1413-8670
dc.description.abstract Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 547-552
dc.language.iso eng
dc.publisher Brazilian Society of Infectious Diseases
dc.relation.ispartof Brazilian Journal of Infectious Diseases
dc.rights Acesso aberto
dc.subject HCV en
dc.subject HIV en
dc.subject infection en
dc.subject reverse transcriptase polymerase chain reaction en
dc.subject blood banks en
dc.title Magnetic bead technology for viral RNA extraction from serum in blood bank screening en
dc.type Artigo
dc.contributor.institution Associação Beneficente de Coleta de Sangue
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Associação Beneficente de Coleta de Sangue
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUnifesp UNIFESP
dc.identifier.file S1413-86702011000600008.pdf
dc.identifier.scielo S1413-86702011000600008
dc.identifier.doi 10.1590/S1413-86702011000600008
dc.description.source SciELO


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