Magnetic bead technology for viral RNA extraction from serum in blood bank screening

Magnetic bead technology for viral RNA extraction from serum in blood bank screening

Author Albertoni, Guilherme Ambrozio Autor UNIFESP Google Scholar
Arnoni, Carine Prisco Google Scholar
Araujo, Patricia Regina Barboza Google Scholar
Andrade, Sheila Siqueira Autor UNIFESP Google Scholar
Carvalho, Fabrício Oliveira Google Scholar
Girão, Manoel João Batista Castello Autor UNIFESP Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Barreto, José Augusto Google Scholar
Institution Associação Beneficente de Coleta de Sangue
Universidade Federal de São Paulo (UNIFESP)
Abstract Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.
Keywords HCV
reverse transcriptase polymerase chain reaction
blood banks
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2011-12-01
Published in Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 6, p. 547-552, 2011.
ISSN 1413-8670 (Sherpa/Romeo)
Publisher Brazilian Society of Infectious Diseases
Extent 547-552
Access rights Open access Open Access
Type Article
SciELO ID S1413-86702011000600008 (statistics in SciELO)

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