Spatial exploration of Streptococcus pneumoniae clonal clustering in São Paulo, Brazil

Spatial exploration of Streptococcus pneumoniae clonal clustering in São Paulo, Brazil

Autor Mouro, Amilton Autor UNIFESP Google Scholar
Kiffer, Carlos Roberto Veiga Autor UNIFESP Google Scholar
Koga, Paula C.m Autor UNIFESP Google Scholar
Monteiro, Antonio M.v Google Scholar
Camargo, Eduardo Celso Gerbi Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Hospital Israelita Albert Einstein
GC-2 Gestão do Conhecimento Científico Ltd
Instituto Nacional de Pesquisas Espaciais Department of Image Processing
Resumo OBJECTIVES: To examine the spatial distribution of Streptococcus pneumoniae and its clonal patterns collected between 2002 and 2006 in São Paulo, Brazil. METHODS: As part of an observational study in São Paulo city, Brazil, S. pneumoniae isolates routinely cultured from blood, respiratory specimens, or cerebrospinal and other profound fluids were selected. Additionally, only isolates with either penicillin (PEN) intermediate (I) or resistant (R) status on routine antibiogram were included, in order to obtain a higher probability of clonal isolates. A single I/R S. pneumoniae isolate per patient was included and submitted to genotypic determination by pulsed field gel electrophoresis (PFGE). Minimum inhibitory concentrations (MICs) were determined for the isolates by Etest® to PEN and other antimicrobials. Each isolate was geocoded in a digital map. The Kernel function and ratio methods between total isolates vs. clones were used in order to explore possible cluster formations. RESULTS: Seventy-eight (78) S. pneumoniae community isolates from two major outpatient centers in São Paulo, Brazil, were selected from the databank according to their penicillin susceptibility profile, i.e. R or I to penicillin assessed by oxacillin disc diffusion. Of these, 69 were submitted to PFGE, 65 to MIC determination, and 48 to spatial analytical procedures. Preliminary spatial analysis method showed two possible cluster formation located in southwest and southeast regions of the city. CONCLUSION: Further analyses are required for precisely determining the existence of S. pneumoniae clusters and their related risk factors. Apparently there is a specific transmission pattern of S. pneumoniae clones within certain regions and populations. GIS and spatial methods can be applied to better understand epidemiological patterns and to identify target areas for public health interventions.
Palavra-chave Streptococcus pneumoniae
penicillin resistance
drug resistance, bacterial
molecular epidemiology
Idioma Inglês
Data de publicação 2011-10-01
Publicado em Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 5, p. 462-466, 2011.
ISSN 1413-8670 (Sherpa/Romeo, fator de impacto)
Publicador Brazilian Society of Infectious Diseases
Extensão 462-466
Fonte http://dx.doi.org/10.1590/S1413-86702011000500008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000295936600008
SciELO S1413-86702011000500008 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6646

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