New multilocus sequence typing of MRSA in São Paulo, Brazil

New multilocus sequence typing of MRSA in São Paulo, Brazil

Autor Carmo, Mirian Silva do Autor UNIFESP Google Scholar
Inoue, Fernanda Autor UNIFESP Google Scholar
Andrade, Soraya Sgambatti Autor UNIFESP Google Scholar
Paschoal, Luiz Autor UNIFESP Google Scholar
Silva, Fernanda M. Autor UNIFESP Google Scholar
Oliveira, Vitor Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Centro Universitário Fundação Instituto de Ensino para Osasco
Resumo An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were characterized as SCCmec, mecA and PVL by PCR, pulsed-field gel electrophoresis (PFGE) profile and molecular sequence typing (MLST) genotyping. The isolates presented type IV SCCmec, and none proved to be positive for PVL. The isolates showed a PFGE profile similar to the pediatric clone. MLST genotyping demonstrated that the isolates belonged to clonal complex 5 (CC5), showing a new yqiL allele gene, resulting in a new sequence typing (ST) (1176). Our results showed that strains of MRSA carrying a new ST are emerging in community and nosocomial infections, including bacteremia, in São Paulo, Brazil.
Palavra-chave Methicillin-resistant Staphylococcus aureus
Molecular sequence typing
Resistance
Molecular typing
Idioma Inglês
Data de publicação 2011-10-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 10, p. 1013-1017, 2011.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1013-1017
Fonte http://dx.doi.org/10.1590/S0100-879X2011007500114
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000295721600006
SciELO S0100-879X2011001000006 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/6625

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