Inactivation of capsaicin-sensitive nerves reduces pulmonary remodeling in guinea pigs with chronic allergic pulmonary inflammation

Mostrar registro simples Prado, Carla Máximo [UNIFESP] Da Rocha, G.z. Leick-Maldonado, Edna Aparecida Starling, Claudia Miranda Capelozzi, Vera Luiza Martins, Milton Arruda Tibério, Iolanda de Fátima Lopes Calvo 2015-06-14T13:42:55Z 2015-06-14T13:42:55Z 2011-02-01
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 2, p. 130-139, 2011.
dc.identifier.issn 0100-879X
dc.description.abstract Pulmonary remodeling is an important feature of asthma physiopathology that can contribute to irreversible changes in lung function. Although neurokinins influence lung inflammation, their exact role in the extracellular matrix (ECM) remodeling remains to be determined. Our objective was to investigate whether inactivation of capsaicin-sensitive nerves modulates pulmonary ECM remodeling in animals with chronic lung inflammation. After 14 days of capsaicin (50 mg/kg, sc) or vehicle administration, male Hartley guinea pigs weighing 250-300 g were submitted to seven inhalations of increasing doses of ovalbumin (1, 2.5, and 5 mg/mL) or saline for 4 weeks. Seventy-two hours after the seventh inhalation, animals were anesthetized and mechanically ventilated and the lung mechanics and collagen and elastic fiber content in the airways, vessels and lung parenchyma were evaluated. Ovalbumin-exposed animals presented increasing collagen and elastic fiber content, respectively, in the airways (9.2 ± 0.9; 13.8 ± 1.2), vessels (19.8 ± 0.8; 13.4 ± 0.5) and lung parenchyma (9.2 ± 0.9; 13.8 ± 1.2) compared to control (P < 0.05). Capsaicin treatment reduced collagen and elastic fibers, respectively, in airways (1.7 ± 1.1; 7.9 ± 1.5), vessels (2.8 ± 1.1; 4.4 ± 1.1) and lung tissue (2.8 ± 1.1; 4.4 ± 1.1) of ovalbumin-exposed animals (P < 0.05). These findings were positively correlated with lung mechanical responses to antigenic challenge (P < 0.05). In conclusion, inactivation of capsaicin-sensitive nerve fibers reduces pulmonary remodeling, particularly collagen and elastic fibers, which contributes to the attenuation of pulmonary functional parameters. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 130-139
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject Collagen en
dc.subject Elastic fibers en
dc.subject Capsaicin en
dc.subject Chronic lung inflammation en
dc.title Inactivation of capsaicin-sensitive nerves reduces pulmonary remodeling in guinea pigs with chronic allergic pulmonary inflammation en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade de São Paulo Departamento de Clínica Médica
dc.description.affiliation Universidade de São Paulo Faculdade de Medicina Departamento de Patologia
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Ciências Biológicas
dc.description.affiliationUnifesp UNIFESP, Depto. de Ciências Biológicas
dc.identifier.file S0100-879X2011000200007.pdf
dc.identifier.scielo S0100-879X2011000200007
dc.identifier.doi 10.1590/S0100-879X2010007500151
dc.description.source SciELO
dc.identifier.wos WOS:000287139000007

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