HIV-1 drug resistance genotypic profiles in children with undetectable plasma viremia during antiretroviral therapy

HIV-1 drug resistance genotypic profiles in children with undetectable plasma viremia during antiretroviral therapy

Author Angelis, Daniela Souza Araújo de Google Scholar
Tateno, Adriana Fumie Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Succi, Regina Célia de Menezes Autor UNIFESP Google Scholar
Pannuti, Claudio Sergio Google Scholar
Gouvêa, Aida de Fátima Thomé Barbosa Autor UNIFESP Google Scholar
Machado, Daisy Maria Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Treatment of HIV-1 infection with highly active antiretroviral therapy has led to sustained viral suppression in the plasma in a large number of children. However, studies have suggested that the integrated provirus in resting CD4+ T lymphocytes could be a source of reactivatable virus and maintain drug-resistant virus. We evaluated the resistance-related mutations in children receiving antiretroviral therapy with prolonged viral suppression. Thirty-two peripheral blood mononuclear cell samples from 16 children with viral loads that had been below detection limits for at least 12 months were obtained at two different time points and the DNAs sequenced. The median CD4 cell count was 1,016 cells/mm³ (347-2,588) and 938 cells/mm³ (440-3,038) at the first and second time points, respectively. The median follow-up time was 15 months (9-27). Six (37.5%) and seven (43.75%) of the 16 patients showed at least one NRTI-associated mutation in the first and second samples, respectively. Two out of 16 (12.5%) had an NNRTI-associated mutation at the first time point and three out of 16 (18.75%) at the second. In addition, 14 out of 16 (87.5%) had at least one PI-associated mutation at both time points. Despite plasma HIV-1 RNA suppression for at least 12 months, resistance-related mutations from previous antiretroviral failures could still be detected in archival virus. Furthermore, viral evolution occurred at the reverse transcriptase region in spite of viral suppression to levels below 400 copies/mL. Persistence of archival resistant virus may be relevant when considering future treatment options.
Keywords children
HIV-1
prolonged viral suppression
antiretroviral therapy
antiretroviral resistance
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-02-01
Published in Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 1, p. 60-65, 2011.
ISSN 1413-8670 (Sherpa/Romeo, impact factor)
Publisher Brazilian Society of Infectious Diseases
Extent 60-65
Origin http://dx.doi.org/10.1590/S1413-86702011000100011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288385100011
SciELO ID S1413-86702011000100011 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6266

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