Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis

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dc.contributor.author Nishiwaki-Dantas, Maria Cristina
dc.contributor.author Abreu, Mariza Toledo de [UNIFESP]
dc.contributor.author Melo, Cynthia Mendonça de
dc.contributor.author Romero, Ivana Lopes
dc.contributor.author Belfort, Rubens Neto [UNIFESP]
dc.contributor.author Dantas, Paulo Elias Correa
dc.date.accessioned 2015-06-14T13:42:49Z
dc.date.available 2015-06-14T13:42:49Z
dc.date.issued 2011-01-01
dc.identifier http://dx.doi.org/10.1590/S1807-59322011001200003
dc.identifier.citation Clinics. Faculdade de Medicina / USP, v. 66, n. 12, p. 2013-2018, 2011.
dc.identifier.issn 1807-5932
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/6208
dc.description.abstract OBJECTIVES: To identify Chlamydia trachomatis via polymerase chain reaction and a direct fluorescent antibodyassay in patients with vernal keratoconjunctivitis while comparing the efficacies of both tests for detectingChlamydia trachomatis in these conditions. METHODS: Conjunctival scraping samples were obtained from 177 patients who were divided into two groups: avernal keratoconjunctivitis group (group A) and a control group (group B). The polymerase chain reaction and adirect fluorescent antibody assay were performed. Sensitivity, specificity, receiver operating characteristic curves,and areas under the curve were calculated for both tests in groups A and B. Receiver operating characteristic curveswere plotted using a categorical variable with only two possible outcomes (positive and negative). RESULTS: Statistical analysis revealed a significant association between vernal keratoconjunctivitis and Chlamydia trachomatis infection detected by a direct fluorescent antibody assay with high sensitivity and specificity. Allpatients in group A with positive polymerase chain reactions also presented with positive direct fluorescentantibody assays. CONCLUSION: The association between vernal keratoconjunctivitis and Chlamydia trachomatis infection wasconfirmed by positive direct fluorescent antibody assays in 49.4% of vernal keratoconjunctivitis patients and bypositive polymerase chain reactions in 20% of these patients. The direct fluorescent antibody assay detectedChlamydia trachomatis in a higher number of patients than did the polymerase chain reaction. Although thediagnosis of trachoma is essentially clinical, the disease may not be detected in vernal keratoconjunctivitis patients.Due to the high frequency of chlamydial infection detected in patients with vernal keratoconjunctivitis, we suggestconsidering routine laboratory tests to detect Chlamydia trachomatis in patients with severe and refractory allergicdisease. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 2013-2018
dc.language.iso eng
dc.publisher Faculdade de Medicina / USP
dc.relation.ispartof Clinics
dc.rights Acesso aberto
dc.subject Vernal keratoconjunctivitis en
dc.subject Chlamydia trachomatis en
dc.subject Polymerase chain reaction en
dc.subject Direct fluorescentantibody assay en
dc.title Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis en
dc.type Artigo
dc.contributor.institution Santa Casa of São Paulo Corneal and External Disease Service Department of Ophthalmology
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Sorocaba Eye Hospital
dc.description.affiliation Santa Casa of São Paulo Corneal and External Disease Service Department of Ophthalmology
dc.description.affiliation Federal University of São Paulo Department of Ophthalmology
dc.description.affiliation Sorocaba Eye Hospital
dc.description.affiliationUnifesp UNIFESP, Department of Ophthalmology
dc.identifier.file S1807-59322011001200003.pdf
dc.identifier.scielo S1807-59322011001200003
dc.identifier.doi 10.1590/S1807-59322011001200003
dc.description.source SciELO
dc.identifier.wos WOS:000298331400003



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