Respiratory mechanics do not always mirror pulmonary histological changes in emphysema

Respiratory mechanics do not always mirror pulmonary histological changes in emphysema

Autor Anciaes, Adriana Martins Google Scholar
Olivo, Clarice Rosa Google Scholar
Prado, Carla Máximo Autor UNIFESP Google Scholar
Kagohara, Keila H. Google Scholar
Pinto, Tatiana da Silva Google Scholar
Moriya, Henrique T. Google Scholar
Mauad, Thais Google Scholar
Martins, Mílton de Arruda Google Scholar
Lopes, Fernanda Degobbi Tenorio Quirino dos Santos Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo OBJECTIVE: To verify the accordance of functional and morphometric parameters during the development of emphysema. METHODS: BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such as airway resistance, tissue damping, and tissue elastance, were analyzed. To evaluate the structural changes and possible mechanisms involved in this disease, we measured the mean linear intercept, the volume proportions of elastic and collagen fibers, the number of macrophages, the numbers of cells expressing metalloprotease 12 and 8-isoprostane in lung parenchyma. RESULTS: We only observed decreases in tissue elastance and tissue damping on the 28th day, with a concomitant increase in the mean linear intercept, indicating the presence of emphysema. However, only the mean linear intercept values remained increased until the 40th day. The volume proportion of collagen fibers was increased from the 15th day to the 40th day, whereas the volume proportion of elastic fibers was only increased on the 40th day. The number of macrophages increased beginning on the 1st day. The expression of metalloproteinase 12 was increased from the 3rd day until the 40th day. However, 8-isoprostane expression was only increased on the 1st and 3rd days. CONCLUSIONS: In this study, morphometric parameters were found to be more reliable for detecting the presence of emphysema than the functional parameters measured by respiratory mechanics. Further investigations are necessary to understand how the extracellular matrix remodeling observed in the lung parenchyma could be involved in this process.
Assunto Emphysema
Animal model
Mean linear intercept
Respiratory mechanics
Parenchyma remodeling
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Laboratorios de Investigacao Medica do Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (LIM/HC)
Data 2011-01-01
Publicado em Clinics. Faculdade de Medicina / USP, v. 66, n. 10, p. 1797-1803, 2011.
ISSN 1807-5932 (Sherpa/Romeo, fator de impacto)
Editor Faculdade de Medicina / USP
Extensão 1797-1803
Fonte http://dx.doi.org/10.1590/S1807-59322011001000020
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000296015500020
SciELO S1807-59322011001000020 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/6120

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