Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Alternative title Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate
Author Zanutto, Talita Corrêa Nazareth Google Scholar
Zanutto, Conrado Planas Google Scholar
Maass, Danielle Autor UNIFESP Google Scholar
Souza, Antônio Augusto Ulson de Google Scholar
Souza, Selene Maria de Arruda Guelli Ulson de Google Scholar
Abstract This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (KLa) concerning volumetric productivity, and the effect of agitation (150–300 rpm) and aeration (0.5–1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product.
Keywords Biosurfactant
Surfactin
Brewery waste
Bioreactor
Volumetric oxygen mass transfer coefficient
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number #2019/07659-4
Date 2021-03-15
ISSN 2213-3437 (Sherpa/Romeo)
Publisher Elsevier
Origin https://www.sciencedirect.com/science/article/pii/S2213343721003493
Access rights Closed access
Type Article
URI https://repositorio.unifesp.br/handle/11600/60836

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