Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection

Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection

Autor Eickhoff, Christopher S Google Scholar
Giddings, Olivia K Google Scholar
Yoshida, Nobuko Autor UNIFESP Google Scholar
Hoft, Daniel F Google Scholar
Instituição Saint Louis University Department of Molecular Microbiology
Universidade Federal de São Paulo (UNIFESP)
Resumo The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
Assunto Trypanosoma cruzi
mucosal immunity
vaccines
Idioma Inglês
Data 2010-08-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 5, p. 687-691, 2010.
ISSN 0074-0276 (Sherpa/Romeo)
Editor Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 687-691
Fonte http://dx.doi.org/10.1590/S0074-02762010000500015
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762010000500015 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/5853

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