Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?

Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?

Author Ferreira, Cecília da Silva Autor UNIFESP Google Scholar
Carvalho, Kátia Cândido Autor UNIFESP Google Scholar
Maganhin, Carla Cristina Autor UNIFESP Google Scholar
Paiotti, Ana Paula Ribeiro Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares Júnior, José Maria Autor UNIFESP Google Scholar
Abstract Melatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.
Keywords Melatonin
Female rats
Continuous light
Apoptosis
xmlui.dri2xhtml.METS-1.0.item-coverage Dordrecht
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 09/51754-0
CNPq: 137442/2009-2
Date 2016
Published in Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.
ISSN 1360-8185 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 155-162
Origin https://doi.org/10.1007/s10495-015-1195-0
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000368176900004
URI https://repositorio.unifesp.br/handle/11600/58519

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