Can gamma-radiation modulate hemagglutinating and anticoagulant activities of PpyLL, a lectin from Phthirusa pyrifolia?

Can gamma-radiation modulate hemagglutinating and anticoagulant activities of PpyLL, a lectin from Phthirusa pyrifolia?

Author Costa, Romero M. P. B. Google Scholar
Campos Albuquerque, Wendell Wagner Google Scholar
Silva, Mariana C. C. Autor UNIFESP Google Scholar
de Paula, Raiana Apolinario Google Scholar
Melo, Mychely Sheila Google Scholar
Oliva, Maria L. V. Autor UNIFESP Google Scholar
Figueiredo Porto, Ana Lucia Google Scholar
Abstract Blood coagulation and platelet-dependent primary homeostasis are important defense mechanisms against bleeding and novel inhibitors have been researched to obtain pharmacological and clinical applications. In this work, the PpyLL, a lectin obtained from Phthirusa pyrifolia, was characterized in terms of its molecular structure and biological functions (anticoagulant, antiplatelet agreggation and hemagglutinating activities) in presence or absence of Gamma radiation exposure. Results revealed a lectin with secondary-structure content by approximately 49% of beta-sheet, 20% of beta-turn and 31% of disordered structure. Irradiation effect demonstrated possible different sites of function by lectin on anticoagulant and hemagglutinating activities, once a decrease about 80% was observed when compared the activities under 0.5 kGy of exposition to gamma radiation. An emphatic discussion about the use of gamma radiation as a possible modulator of the lectin activity was made, and once the ionizing radiation affected differently the anticoagulation and hemagglutinating activities, we speculated that the results are determined by selective molecular damages in different binding sites. PpyLL biological activities and gamma radiation modulation could be considered for future researches in biomedical field aiming possible medical applications. (C) 2017 Elsevier B.V. All rights reserved.
Keywords Activity modulated
Coagulation
Platelet aggregation
Binding site
Gamma radiation
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Date 2017
Published in International Journal Of Biological Macromolecules. Amsterdam, v. 104, p. 125-136, 2017.
ISSN 0141-8130 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 125-136
Origin http://dx.doi.org/10.1016/j.ijbiomac.2017.06.007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000412959300014
URI https://repositorio.unifesp.br/handle/11600/58295

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