CA88, a nuclear repetitive DNA sequence identified in Schistosoma mansoni, aids in the genotyping of nine Schistosoma species of medical and veterinary importance

CA88, a nuclear repetitive DNA sequence identified in Schistosoma mansoni, aids in the genotyping of nine Schistosoma species of medical and veterinary importance

Autor Bahia, Diana Autor UNIFESP Google Scholar
Rodrigues, Nilton B Google Scholar
Araújo, Flávio Marcos G Google Scholar
Romanha, Álvaro José Google Scholar
Ruiz, Jerônimo C Google Scholar
Johnston, David A Google Scholar
Oliveira, Guilherme Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Instituto de Pesquisas René Rachou-Fiocruz
The Natural History Museum Department of Zoology
Santa Casa de Belo Horizonte Programa de Pós-Graduação e Pesquisa
Universidade Federal de Ouro Preto Escola de Farmácia Laboratório de Pesquisas Clínicas
Resumo CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA microsatellite, identified as SmBr18. Both PCR and BLAST analysis have been used to analyse and confirm the CA88 sequence in other S. mansoni sequences in the public database. PCR-acquired nuclear repetitive DNA sequence profiles from nine Schistosoma species were used to classify this organism into four genotypes. Included among the nine species analysed were five sequences of both African and Asian lineages that are known to infect humans. Within these genotypes, three of them refer to recognised species groups. A panel of four microsatellite loci, including SmBr18 and three previously published loci, has been used to characterise the nine Schistosoma species. Each species has been identified and classified based on its CA88 DNA fingerprint profile. Furthermore, microsatellite sequences and intra-specific variation have also been observed within the nine Schistosoma species sequences. Taken together, these results support the use of these markers in studying the population dynamics of Schistosoma isolates from endemic areas and also provide new methods for investigating the relationships between different populations of parasites. In addition, these data also indicate that Schistosoma magrebowiei is not a sister taxon to Schistosoma mattheei, prompting a new designation to a basal clade.
Assunto genotypes
microsatellite
Schistosoma
species
population dynamics
Idioma Inglês
Data 2010-07-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 4, p. 391-397, 2010.
ISSN 0074-0276 (Sherpa/Romeo)
Editor Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 391-397
Fonte http://dx.doi.org/10.1590/S0074-02762010000400008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762010000400008 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/5825

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