Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

Differential cytotoxic effects on odontoblastic cells induced by self-adhesive resin cements as a function of the activation protocol

Author Perlatti D'Alpino, Paulo Henrique Google Scholar
Moura, Gioconda Emanuella Diniz de Dantas Autor UNIFESP Google Scholar
de Arruda Barbosa, Silvana Coelho Google Scholar
Marques, Lygia de Azevedo Google Scholar
Eberlin, Marcos Nogueira Google Scholar
Nascimento, Fabio Dupart Google Scholar
Tersariol, Ivarne Luis dos Santos Autor UNIFESP Google Scholar
Abstract Objectives. To evaluate the cytotoxic effects of exposing odontoblast cells to a variety of commercial self-adhesive cements polymerized using different activation modes. Methods. Five cements: MaxCem Elite (MAX), Bifix SE (BSE), G-Cem LinkAce (GCE), Clearfil SA Luting (CAS), and RelyX U200 (U200) were mixed, dispensed into molds, and distributed in groups, according to polymerization protocols: immediate photoactivation; delayed photoactivation (10 min self-curing plus light-activation); and chemical activation (no light exposure). Immortalized rat odontoblast cells (MDPC-23) were cultured. Cell viability was assessed by Trypan Blue staining and total cell death was assessed by annexin V-APC/7-AAD double staining and flow cytometry. Volatilized compounds from polymerized specimens of cements were evaluated by gas chromatography/mass spectrometry (GC-MS). Data was analyzed with 2-way ANOVA/Tukey tests (alpha = 0.05). Results. Exposure to all of the cements tested significantly reduced the cell viability, irrespective of the activation protocol (p < 0.05). The least harmful cements were CSA and U200. Total death of cells significantly increased when exposed to BSE, GCE, and MAX, especially when chemically activated (p < 0.05). Characteristic apoptotic cells increased after exposure to cements, mainly for MAX, regardless of the activation mode. Chemical activation of MAX also induced necrosis. Moreover, GCE and MAX exhibited higher percentages of late apoptotic/dead cells. Chromatograms revealed 28 compounds released from the cements tested, some of them with known carcinogenic effects. Selection of self-adhesive cements and polymerization protocols affect the cytotoxicity and cell viability of odontoblastic cells. Clinical significance. Despite the simplified cementation protocol, care is needed when cementing indirect restorations with self-adhesive cements, especially on recently exposed dentin. This category of material may cause differential cytotoxic effects and should be considered when selecting a cement. This is particularly true in clinical cases of light attenuation, where the polymerization depends on chemical activation, inducing higher cytotoxic damages when using some of the cements tested. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
Keywords Self-adhesive resin cements
Odontoblastic cells
Cytotoxicity
Total death cell
Gas Chromatography
Mass spectrometry
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor FAPESP
Grant number FAPESP: 13/05822-9
Date 2017
Published in Dental Materials. Oxford, v. 33, n. 12, p. 1402-1415, 2017.
ISSN 0109-5641 (Sherpa/Romeo, impact factor)
Publisher Elsevier Sci Ltd
Extent 1402-1415
Origin http://dx.doi.org/10.1016/j.dental.2017.09.011
Access rights Closed access
Type Article
Web of Science ID WOS:000417082200010
URI https://repositorio.unifesp.br/handle/11600/58102

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