High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion

High Risk HPV E6/E7 Oncoprotein Expression in Women with High Grade Squamous Intraepithelial Lesion

Author Cordeiro Valenca, Jefferson Elias Google Scholar
Goncalves, Ana Katherine Google Scholar
Cotrim Guerreiro da Silva, Ismael Dale Autor UNIFESP Google Scholar
Eleuterio Junior, Jose Google Scholar
da Silva, Terezinha Tenerio Google Scholar
Bruneska, Danyelly Google Scholar
de Alencar Ximenes, Ricardo Arraes Google Scholar
Abstract Purpose To correlate the expression of high-risk HPV E6 mRNA with pap smear, colposcopy, and biopsy results in women with high grade squamous intraepithelial lesion (HSIL). Methods A cross-sectional study was performed on women referred for primary care services after cytological diagnosis of HSIL. We evaluated the expression of E6/E7 mRNA of HPV types 16,18,31,33, and 45 and correlated the results with those of Pap smear, colposcopy, and biopsy. For amplification/detection of mRNA E6/E7 we used NucliSENSEasyQ kit to detect HPV mRNA by polymerase chain reaction with primers/probes for HPV types 16, 18, 31, 33, and 45. Results Out of 128 valid tests, the results of 30 (23.4%) tests were negative and 98 (70%) tests were positive. Only one type of HPV was detected in 87.7% of the E6/E7 mRNA positive cases. HPV16 was detected in 61.2% of the cases, followed by HPV33 (26.5%), HPV31 (17.3%), HPV18 (10%), and HPV45 (4.08%). Pap smear tests revealed that the E6/E7 test was positive in 107 (83.8%) women with atypical squamous cells high grade (ASC-H), HSIL, or higher. The E6/E7 test was positive in 69 (57.5%) specimens presenting negative cytology results. When analyzing the association with colposcopy results, the frequency of positive E6/E7 results increased with the severity of the injury, ranging from 57.1% in women without colposcopy-detected injury to 86.5% in those with higher levels of colposcopy findings. Of the 111 women who underwent biopsy and E6/E7 testing, the E6/E7 test was positive in 84.7% of the women who presented with lesions of cervical intraepithelial neoplasia (CIN) grade 2 or higher. Finally, 41.2% of women with a negative biopsy presented a positive E6/E7 test. Conclusions E6/E7 mRNA expression was higher in women with HSIL and CIN grade 2 or higher.
Keywords colposcopy
HPV
mRNA
Pap smear
women
xmlui.dri2xhtml.METS-1.0.item-coverage Rio De Janeiro Rj
Language English
Date 2016
Published in Revista Brasileira De Ginecologia E Obstetricia. Rio De Janeiro Rj, v. 38, n. 3, p. 154-159, 2016.
ISSN 0100-7203 (Sherpa/Romeo, impact factor)
Publisher Federacao Brasileira Soc Ginecologia & Obstetricia-Febrasgo
Extent 154-159
Origin http://dx.doi.org/10.1055/s-0036-1580713
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000410243300007
SciELO ID S0100-72032016000300154 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/57943

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