Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus

Cellulolytic and proteolytic ability of bacteria isolated from gastrointestinal tract and composting of a hippopotamus

Author da Cruz Ramos, Geomarcia Feitosa Autor UNIFESP Google Scholar
Ramos, Patricia Locosque Google Scholar
Zambrano Passarini, Michel Rodrigo Autor UNIFESP Google Scholar
Vieira Silveira, Marghuel A. Autor UNIFESP Google Scholar
Okamoto, Debora Noma Autor UNIFESP Google Scholar
Goncalves de Oliveira, Lilian Caroline Autor UNIFESP Google Scholar
Zezzo, Larissa Vieira Autor UNIFESP Google Scholar
Marem, Alyne Autor UNIFESP Google Scholar
Santos Rocha, Rafael Costa Autor UNIFESP Google Scholar
da Cruz, Joao Batista Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
de Vasconcellos, Suzan Pantaroto Autor UNIFESP Google Scholar
Abstract The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternative sources. In this context, the goal of this study was the investigation of cellulolytic and proteolytic abilities of bacteria isolated from the gastrointestinal tract of a hippopotamus as well as from its composting process. It is important to highlight that hippopotamus gastrointestinal samples were a non-typical sources of efficient hydrolytic bacteria with potential for application in biotechnological industries, like biofuel production. Looking for this, a total of 159 bacteria were isolated, which were submitted to qualitative and quantitative enzymatic assays. Proteolytic analyzes were conducted through the evaluation of fluorescent probes. Qualitative assays for cellulolytic abilities revealed 70 positive hits. After quantitative analyzes, 44 % of these positive hits were selected, but five (5) strains showed cellulolytic activity up to 11,8 FPU/mL. Regarding to proteolytic activities, six (6) strains showed activity above 10 %, which overpassed results described in the literature. Molecular analyzes based on the identification of 16S rDNA, revealed that all the selected bacterial isolates were affiliated to Bacillus genus. In summary, these results strongly indicate that the isolated bacteria from a hippopotamus can be a potential source of interesting biocatalysts with cellulolytic and proteolytic activities, with relevance for industrial applications.
Keywords Protease
Cellulase
Screening
Biofuel
Bacillus
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Brazilian research agency (FAPESP)
Brazilian research agency (CAPES)
Brazilian research agency (CNPq)
Grant number FAPESP: 2010/51992-5
CNPq: 475166/2013-2
Date 2016
Published in Amb Express. London, v. 6, p. -, 2016.
ISSN 2191-0855 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent -
Origin http://dx.doi.org/10.1186/s13568-016-0188-x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000371385600001
URI https://repositorio.unifesp.br/handle/11600/57905

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