Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice

Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice

Author Pacheco-Costa, R. Autor UNIFESP Google Scholar
Davis, H. M. Google Scholar
Atkinson, E. G. Google Scholar
Katchburian, E. Autor UNIFESP Google Scholar
Plotkin, L. I. Google Scholar
Reginato, R. D. Autor UNIFESP Google Scholar
Abstract Objective: To investigate whether osteocytic connexin 43 (Cx43) is required for the bone response to intermittent PTH administration, and whether the connexin is involved in maintaining the bone matrix. Methods: Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl); DMP1-8kb-Cre) daily (100 mu g/kg/d) for 14 days. Results: Cx43(fl/fl); DMP1-8kb-Cre mice have no difference in body weight and BMD from 1 to 4 months of age. Intermittent PTH administration increased BMD and BV/TV and induced a similar increase in type I collagen, alkaline phosphatase, runx2, osteocalcin, and bone sialoprotein expression in mice from both genotypes. On the other hand, osteocytic deletion of Cx43 did not alter mRNA levels of glycosaminoglycans, proteoglycans, collagens and osteoblast-related genes. In addition, expression of collagens assessed by immunohistochemistry was not affected by deleting osteocytic Cx43. However, PTH administration increased type II collagen only in Cx43(fl/fl) control mice, whereas hormone increased type I collagen expression only in Cx43(fl/fl); DMP1-8kb-Cre mice. Furthermore, PTH increased maturity of collagen fibers in control, but not in Cx43-deficient mice. Conclusion: Expression of Cx43 in osteocytes is dispensable for bone anabolism induced by intermittent PTH administration; but it can modulate, at least in part, the effect of PTH on the bone matrix environment.
Keywords Bone Matrix
Connexin 43
xmlui.dri2xhtml.METS-1.0.item-coverage Nafplion
Language English
Sponsor IUPUI
Life-Health Sciences Internship Program
CTSI - Clinical and Transitional Sciences Institute Award
Coordination of Improvement of Higher Level Personnel (CAPES), Brazil (PDEE)
National Institutes of Health, USA
National Council for Scientific and Technological Development - CNPq, Brazil
Grant number CAPES PDEE: 1065/11-4
National Institutes of Health: R01-AR053643
Date 2016
Published in Journal Of Musculoskeletal & Neuronal Interactions. Nafplion, v. 16, n. 1, p. 45-57, 2016.
ISSN 1108-7161 (Sherpa/Romeo, impact factor)
Publisher Jmni
Extent 45-57
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372527200008
URI https://repositorio.unifesp.br/handle/11600/57875

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