BmTI-A, a Kunitz type inhibitor from Rhipicephalus microplus able to interfere in vessel formation

BmTI-A, a Kunitz type inhibitor from Rhipicephalus microplus able to interfere in vessel formation

Author Soares, Tatiane S. Autor UNIFESP Google Scholar
Oliveira, Felipe Autor UNIFESP Google Scholar
Torquato, Ricardo J. S. Autor UNIFESP Google Scholar
Sasaki, Sergio D. Google Scholar
Araujo, Mariana S. Autor UNIFESP Google Scholar
Paschoalin, Thaysa Autor UNIFESP Google Scholar
Tanaka, Aparecida S. Autor UNIFESP Google Scholar
Abstract Rhipicephalus microplus is an ectoparasite responsible for transmissions of babesiosis and anaplasmosis causing large losses to livestock production. To survive R. microplus tick produces several active molecules, such as protease inhibitors. This ectoparasite has been described as a rich source of serine protease inhibitors most of them are Kunitz-BPTI members named BmTIs which have no clear function yet. In the present work, we described the expression and functional characterization of rBmTI-A which showed to be similar to the native BmTI-A, a double-headed Kunitz-BPTI inhibitor, capable to inhibit trypsin, human neutrophil elastase (HNE), human plasma kalikrein (HuPK) and human plasmin. rBmTI-A was able to cause a decrease of HUVEC cell viability. Besides, the rBmTI-A showed to be a potent inhibitor of "in vitro" vessel formation. Our results suggested that BmTI-A may participate in the blood acquisition process interfering in the vessel formation during the tick parasite life stage, around 20 days. In conclusion, BmTI-A is a promising molecule to be used in the drug design and development of new method of R. microplus control. (C) 2016 Elsevier B.V. All rights reserved.
Keywords Tick
Rhipicephalus microplus
Kallikrein
Plasmin
Protease inhibitor
Kunitz
Angiogenesis
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundação de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
INCT-Entomologia Molecular, Brazil
PRONEX
Grant number FAPESP: 02/13960-8
FAPESP: 05/03514-9
FAPESP: 12/03657-8
CNPq: 308780/2013-2
Date 2016
Published in Veterinary Parasitology. Amsterdam, v. 219, p. 44-52, 2016.
ISSN 0304-4017 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 44-52
Origin http://dx.doi.org/10.1016/j.vetpar.2016.01.021
Access rights Closed access
Type Article
Web of Science ID WOS:000371943700007
URI https://repositorio.unifesp.br/handle/11600/57770

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