Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients

Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients

Autor Carraro, Emerson Autor UNIFESP Google Scholar
Pasternak, Jacyr Autor UNIFESP Google Scholar
Perosa, Ana Helena Sitta Autor UNIFESP Google Scholar
Siqueira, Itacy Autor UNIFESP Google Scholar
Martino, Marines Dalla Vale Autor UNIFESP Google Scholar
Instituição Hospital Albert Einstein Clinical Laboratory Microbiology Section
Universidade Federal de São Paulo (UNIFESP)
Resumo Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results.
Palavra-chave cytomegalovirus
antigenemia assay
immmunoperoxidase
immunofluorescence
Idioma Inglês
Data de publicação 2010-06-01
Publicado em Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 14, n. 3, p. 322-324, 2010.
ISSN 1413-8670 (Sherpa/Romeo, fator de impacto)
Publicador Brazilian Society of Infectious Diseases
Extensão 322-324
Fonte http://dx.doi.org/10.1590/S1413-86702010000300023
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000281693400023
SciELO S1413-86702010000300023 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/5748

Exibir registro completo




Arquivo

Nome: S1413-86702010000300023.pdf
Tamanho: 736.1KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta