Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection

Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection

Author Nogueira, Paula M. Google Scholar
Assis, Rafael R. Google Scholar
Torrecilhas, Ana C. Autor UNIFESP Google Scholar
Saraiva, Elvira M. Google Scholar
Pessoa, Natlia L. Google Scholar
Campos, Marco A. Google Scholar
Marialva, Eric F. Google Scholar
Rios-Velasquez, Claudia M. Google Scholar
Pessoa, Felipe A. Google Scholar
Secundino, Nagila F. Google Scholar
Rugani, Jeronimo N. Google Scholar
Nieves, Elsa Google Scholar
Turco, Salvatore J. Google Scholar
Melo, Maria N. Google Scholar
Soares, Rodrigo P. Google Scholar
Abstract The immunomodulatory properties of lipophosphoglycans (LPG) from New World species of Leishmania have been assessed in Leishmania infantum and Leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. This glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved Gal(beta 1,4) Man(alpha 1)-PO4 backbone of repeat units. Here, the immunomodulatory activity of LPGs from Leishmania amazonensis, the causative agent of diffuse cutaneous leishmaniasis, was evaluated in two strains from Brazil. One strain (PH8) was originally isolated from the sand fly and the other (Josefa) was isolated from a human case. The ability of purified LPGs from both strains was investigated during in vitro interaction with peritoneal murine macrophages and CHO cells and in vivo infection with Lutzomyia migonei. In peritoneal murine macrophages, the LPGs from both strains activated TLR4. Both LPGs equally activate MAPKs and the NF-kappa B inhibitor p-I.Ba, but were not able to translocate NF-kappa B. In vivo experiments with sand flies showed that both stains were able to sustain infection in L. migonei. A preliminary biochemical analysis indicates intraspecies variation in the LPG sugar moieties. However, they did not result in different activation profiles of the innate immune system. Also those polymorphisms did not affect infectivity to the sand fly.
xmlui.dri2xhtml.METS-1.0.item-coverage San Francisco
Language English
Sponsor Conselho Nacional de Pesquisa e Desenvolvimento of Brazil
Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
Grant number CNPq PAPES VI: 407438/2012-2
CNPq PAPES VI: 407687/2012-2
CNPq PAPES VI: 407743/2012
CNPq PAPES VI: 168260/2014-0
CNPq PROEP: 401975/2012
FAPEMIG: PPM-00163-14
Date 2016
Published in Plos Neglected Tropical Diseases. San Francisco, v. 10, n. 8, p. -, 2016.
ISSN 1935-2735 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent -
Origin http://dx.doi.org/10.1371/journal.pntd.0004848
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000382390800010
URI https://repositorio.unifesp.br/handle/11600/57469

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