Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver

Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver

Author Alves, Claudia C. Autor UNIFESP Google Scholar
Waitzberg, Dan L. Google Scholar
de Andrade, Laila S. Autor UNIFESP Google Scholar
Aguiar, Lais dos Santos Autor UNIFESP Google Scholar
Reis, Milene B. Autor UNIFESP Google Scholar
Guanabara, Camila C. Autor UNIFESP Google Scholar
Junior, Odair A. Autor UNIFESP Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Sala, Priscila Google Scholar
Abstract Background and aims: Non-alcoholic fatty liver disease (NAFLD) is characterized by the presence of fat in hepatocytes because of decreased beta-oxidation and increased lipogenesis. Prebiotics, probiotics, and synbiotic have modulatory effects on intestinal microbiota and may influence the gut-liver axis. Our aim was to evaluate the effects of prebiotic, probiotics, and synbiotic on liver histopathology and gene expression related to b-oxidation and lipogenesis after hypercholesterolemia. Methods: Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (HPC) (60 days). On Day 30 of HPC, rats were subdivided in 5 groups: negative control (NC): without HPC C Gv (distilled water)

positive control (PC): with HPC C Gv (distilled water)

prebiotic (PRE): HPC C Gv with prebiotic (Fiber FOS (R))

probiotic (PRO): HPC C Gv with probiotic strains Gv (Probiatop (R))

and synbiotic (SYN): HPC C Gv with synbiotic (Simbioflora (R)). All rats were sacrificed on Day 30 post-treatment. Blood was collected to verify total serum cholesterol, and liver tissue was sampled to verify histopathological changes and gene expression. Gene expression related to beta-oxidation (PPAR-alpha and CPT-1) and lipogenesis (SREBP-1c, FAS and ME) was evaluated in liver tissue using RT-qPCR. Results: PC had higher cholesterol levels when compared to NC. PRE and SYN rats had lower cholesterol levels than PC. PC rats showed more histopathological changes than NC rats

PRE and SYN rats showed fewer alterations than PC rats. PPAR-alpha was expressed at higher levels in SYN and PC rats compared with PRE and PRO rats. CPT-1 expression was similar in all groups. SREBP-1c was expressed at higher levels in PC rats compared with NC rats

levels were lower in SYN rats compared with PRO rats

levels were lower in PRE rats compared with PC and PRO rats. FAS was expressed at lower levels in PRE rats compared with SYN rats. ME expression was lower in PC rats compared with NC rats. Conclusion: Prebiotic and synbiotic supplementation improve hepatic alterations related to hypercholesterolemia. These changes appear to be mediated by altered expression of genes related to beta-oxidation and lipogenesis.
Keywords cholesterol diet
steatosis
rats
prebiotic
probiotic
synbiotic
gene expression
xmlui.dri2xhtml.METS-1.0.item-coverage Lausanne
Language English
Sponsor FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
Grant number FAPESP: 2011/50289-1
Date 2017
Published in Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
ISSN 1664-302X (Sherpa/Romeo, impact factor)
Publisher Frontiers Media Sa
Extent -
Origin http://dx.doi.org/10.3389/fmicb.2017.02010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000413105800001
URI https://repositorio.unifesp.br/handle/11600/57139

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