Analysis of the Yeast Peptidome and Comparison with the Human Peptidome

Analysis of the Yeast Peptidome and Comparison with the Human Peptidome

Author Dasgupta, Sayani Google Scholar
Yang, Ciyu Google Scholar
Castro, Leandro M. Google Scholar
Tashima, Alexandre Keiji Autor UNIFESP Google Scholar
Ferro, Emer S. Google Scholar
Moir, Robyn D. Google Scholar
Willis, Ian M. Google Scholar
Fricker, Lloyd D. Google Scholar
Abstract Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeast peptides are similar to those of the human peptidome in average size and amino acid composition. Inhibition of proteasome activity with either bortezomib or epoxomicin led to decreased levels of some yeast peptides, suggesting that these peptides are generated by the proteasome. Approximately 30% of the yeast peptides correspond to the N- or C-terminus of the protein

the human peptidome is also highly represented in N- or C-terminal protein fragments. Most yeast and humans peptides are derived from a subset of abundant proteins, many with functions involving cellular metabolism or protein synthesis and folding. Of the 75 yeast proteins that give rise to peptides, 24 have orthologs that give rise to human and/or mouse peptides and for some, the same region of the proteins are found in the human, mouse, and yeast peptidomes. Taken together, these results support the hypothesis that intracellular peptides may have specific and conserved biological functions.
xmlui.dri2xhtml.METS-1.0.item-coverage San Francisco
Language English
Sponsor United States National Institutes of Health [R01-DA004494]
Brazilian National Research Council [400944/2014-6, 445363/2014-2, 303135/2011-5, 449390/2014-4]
Financiadora de Estudos e Projetos
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2012/19321-9]
Grant number NIH: R01-DA004494
CNPq: 400944/2014-6
CNPq: 445363/2014-2
CNPq: 303135/2011-5
CNPq: 449390/2014-4
FAPESP: 2012/19321-9
Date 2016
Published in Plos One. San Francisco, v. 11, n. 9, p. -, 2016.
ISSN 1932-6203 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000384328500042

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