Genotoxicity and mutagenicity induced by acute crack cocaine exposure in mice

Genotoxicity and mutagenicity induced by acute crack cocaine exposure in mice

Author Yujra, Veronica Quispe Autor UNIFESP Google Scholar
Moretti, Eduardo Gregolin Autor UNIFESP Google Scholar
Claudio, Samuel Rangel Autor UNIFESP Google Scholar
Dias Silva, Marcelo Jose Google Scholar
Oliveira, Flavia de Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Abstract Context: Crack cocaine is an illicit drug derived from cocaine, in which use and abuse have increased around the world, especially in developing countries. Objectives: The aim of this study was to evaluate genomic damage in multiple organs of mice following acute exposure to crack cocaine. For this purpose, single cell gel (comet) assay in peripheral blood, liver, kidney, and brain cells was performed and micronucleus test for bone narrow and liver cells was also made in this setting. Material and methods: A total of 20 C57BL/10 male mice were distributed into four groups, as follows: 0, 4.5, 9, and 18mg/kg b.w. of crack cocaine dissolved to 1% dimethyl sulfoxide by intraperitoneal (i.p.) route. All animals were sacrificed 24h after i.p. injection. Results: The results showed that crack cocaine induced DNA damage in peripheral blood, and brain cells for higher doses used as depicted by single cell gel (comet) assay data. Analysis of kidney cells showed no genetic damage for all groups tested. The number of micronucleated cells did not increase after crack cocaine exposure in bone narrow or liver cells. Conclusion: In summary, crack cocaine is a genotoxic agent in peripheral blood, liver, and brain cells but not mutagenic in multiple organs of mice.
Keywords 50-36-2
crack cocaine
DNA damage
mice
xmlui.dri2xhtml.METS-1.0.item-coverage Abingdon
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2016
Published in Drug And Chemical Toxicology. Abingdon, v. 39, n. 4, p. 388-391, 2016.
ISSN 0148-0545 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 388-391
Origin http://dx.doi.org/10.3109/01480545.2015.1126843
Access rights Closed access
Type Article
Web of Science ID WOS:000381357000006
URI https://repositorio.unifesp.br/handle/11600/57088

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