Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination

Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination

Author Sicchieri, Leticia Bonfante Google Scholar
Natal, Rodrigo de Andrade Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Abstract The objective of this study is to characterize arterial tissue with and without atherosclerosis by fluorescence lifetime imaging microscopy (FLIM) using Europium Chlortetracycline complex (EuCTc) as fluorescent marker. For this study, twelve rabbits were randomly divided into a control group (CG) and an experimental group (EG), where they were fed a normal and hypercholesterolemic diet, respectively, and were treated for 60 days. Cryosections of the aortic arch specimens were cut in a vertical plane, mounted on glass slides, and stained with Europium (Eu), Chlortetracycline (CTc), Europium Chlortetracycline (EuCTc), and Europium Chlortetracycline Magnesium (EuCTcMg) solutions. FLIM images were obtained with excitation at 405 nm. The average autofluorescence lifetime within plaque depositions was similar to 1.36 ns. Reduced plaque autofluorescence lifetimes of 0.23 and 0.31 ns were observed on incubation with EuCTc and EuCTcMg respectively. It was observed a quenching of collagen, cholesterol and TG emission spectra increasing EuCTc concentration. The drastic reduction in fluorescence lifetimes is due to a resonant energy transfer between collagen, triglycerides, cholesterol and europium complexes, quenching fluorescence.
Keywords Atherosclerosis
FLIM
Europium Chlortetracycline
FRET
Collagen
Cholesterol
Triglycerides
xmlui.dri2xhtml.METS-1.0.item-coverage Dordrecht
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2014/06960-9
Date 2016
Published in International Journal Of Cardiovascular Imaging. Dordrecht, v. 32, n. 10, p. 1595-1604, 2016.
ISSN 1569-5794 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1595-1604
Origin http://dx.doi.org/10.1007/s10554-016-0936-7
Access rights Closed access
Type Article
Web of Science ID WOS:000383831300016
URI https://repositorio.unifesp.br/handle/11600/57033

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