Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema

Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema

Author Games, Ellen Autor UNIFESP Google Scholar
Guerreiro, Marina Autor UNIFESP Google Scholar
Santana, Fernanda Roncon Autor UNIFESP Google Scholar
Pinheiro, Nathalia M. Google Scholar
Oliveira, Emerson Alves de Autor UNIFESP Google Scholar
Lopes, Fernanda D. T. Q. S. Google Scholar
Olivo, Clarice R. Google Scholar
Tiberio, Iolanda F. L. C. Google Scholar
Martins, Milton A. Google Scholar
Lago, Joao Henrique Ghilardi Autor UNIFESP Google Scholar
Prado, Carla Maximo Autor UNIFESP Google Scholar
Abstract Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and inflammation. Natural products, such as monoterpenes, displayed anti-inflammatory and anti-oxidant activities and can be used as a source of new compounds to COPD treatment. Our aim was to evaluate, in an elastase-induced pulmonary emphysema in mice, the effects of and underlying mechanisms of three related natural monoterpenes (p-cymene, carvacrol and thymol) isolated from essential oil from leaves Lippia sidoides Cham. (Verbenaceae). Methods: Mices received porcine pancreatic elastase (PPE) and were treated with p-cymene, carvacrol, thymol or vehicle 30 min later and again on 7th, 14th and 28th days. Lung inflammatory profile and histological sections were evaluated. Results: In the elastase-instilled animals, the tested monoterpenes reduced alveolar enlargement, macrophages and the levels of IL-1 beta, IL-6, IL-8 and IL-17 in bronchoalveolar lavage fluid (BALF), and collagen fibers, MMP-9 and p-65-NF-kappa B-positive cells in lung parenchyma (p < 0.05). All treatments attenuated levels of 8-iso-PGF2 alpha but only thymol was able to reduced exhaled nitric oxide (p < 0.05). Conclusion: Monoterpenes p-cymene, carvacrol and thymol reduced lung emphysema and inflammation in mice. No significant differences among the three monoterpenes treatments were found, suggesting that the presence of hydroxyl group in the molecular structure of thymol and carvacrol do not play a central role in the anti-inflammatory effects.
Keywords COPD
elastase-induced emphysema
monoterpenes
p-cymene
carvacrol
thymol
lung inflammation
xmlui.dri2xhtml.METS-1.0.item-coverage Basel
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Laboratorio de Investigacao Medica, Hospital das Clinicas, Faculdade de Medicina, University of Sao Paulo (LIM)
Grant number CNPq: 300546/2012-2
CNPq: 304465/2012-7
CNPq: 476877/2012-1
CNPq: 306278/2015-4
FAPESP: 2011/51739-0
FAPESP: 2013/02881-4
FAPESP: 2008/55359-5
FAPESP: 2015/11936-2
FAPESP: 2014/25689-4
LIM: LIM20
Date 2016
Published in Molecules. Basel, v. 21, n. 10, p. -, 2016.
ISSN 1420-3049 (Sherpa/Romeo, impact factor)
Publisher Mdpi Ag
Extent -
Origin http://dx.doi.org/10.3390/molecules21101390
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000389917900127
URI https://repositorio.unifesp.br/handle/11600/56953

Show full item record




File

Name: WOS000389917900127.pdf
Size: 4.349Mb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account