Anti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extracts

Anti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extracts

Author Pedrosa, Tatiana do Nascimento Google Scholar
Barros, Aline Oliveira Google Scholar
Nogueira, Jessica Rodrigues Google Scholar
Fruet, Andrea Costa Google Scholar
Rodrigues, Isis Costa Google Scholar
Calcagno, Danielle Queiroz Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
de Souza, Tatiane Pereira Google Scholar
de Moraes Barros, Silvia Berlanga Google Scholar
de Vasconcellos, Marne Carvalho Google Scholar
Araujo da Silva, Felipe Moura Google Scholar
Ferreira Koolen, Hector Henrique Google Scholar
Maria-Engler, Silvya Stuchi Google Scholar
Lima, Emerson Silva Google Scholar
Abstract Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as juca, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of juca trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 +/- 0.8 A mu g/mL) and LFP (IC50 = 16 +/- 0.5 A mu g/mL), was stronger than standard rutin (IC50 = 27.6 +/- 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.
Keywords Libidibia ferrea
Skin aging
Melanogenesis
Tyrosinase
Polyphenols
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2016
Published in Archives Of Dermatological Research. New York, v. 308, n. 9, p. 643-654, 2016.
ISSN 0340-3696 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 643-654
Origin http://dx.doi.org/10.1007/s00403-016-1685-0
Access rights Closed access
Type Article
Web of Science ID WOS:000386067500005
URI https://repositorio.unifesp.br/handle/11600/56861

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