Genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid in multiples organs of Wistar rats

Genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid in multiples organs of Wistar rats

Author Larangeira, Paula Martins Autor UNIFESP Google Scholar
Rosso, Veridiana Vera de Autor UNIFESP Google Scholar
Silva, Victor Hugo Pereira da Autor UNIFESP Google Scholar
Moura, Carolina Foot Gomes de Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Abstract The ionic liquid or melted salt 1-Butyl-3-methylimidazolium is an alternative process to extract natural pigments, such as carotenoids. Lycopene represents 80-90% of total of carotenoids presents in tomatoes and it has been widely studied due its potent antioxidant action. The aim of this study was to evaluate genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid using experimental model in vivo. For this purpose, a total of 20 male Wistar rats were distributed into four groups (n = 5), as follows: control group

received a corresponding amount of corn oil for 7 days by intragastric gavage (i.g.), ionic liquid group, received 10 mg kg(-1) body weight for 7 days by gavage

10 mg carotenoids group, received 10 mg kg(-1) bw dissolved in corn oil for 7 days by gavage and 500 mg carotenoids group, received 500 mg kg(-1) bw dissolved in corn oil for 7 days by gavage. Rat liver treated with ionic liquid exhibited moderate histopathological changes randomly distributed in the parenchyma, such as cytoplasmic eosinophilia, apoptotic bodies, inflammatory infiltrate and focal necrosis. DNA damage was found in peripheral blood and liver cells of rats treated with ionic liquid or carotenoids at 500 mg. An increase of micronucleated cells and 8-OhDG immunopositive cells were also detected in rats treated with carotenoids at 500 mg. In summary, our results demonstrate that recommended dose for human daily intake of carotenoids extracted by ionic liquid did not induce genotoxicity, mutagenicity and cytotoxicity in multiple organs of rats. (C) 2016 Elsevier GmbH. All rights reserved.
Keywords Ionic liquid
Lycopene
Mutagenicity
Genotoxicity
Rat
xmlui.dri2xhtml.METS-1.0.item-coverage Jena
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2016
Published in Experimental And Toxicologic Pathology. Jena, v. 68, n. 10, p. 571-578, 2016.
ISSN 0940-2993 (Sherpa/Romeo, impact factor)
Publisher Elsevier Gmbh, Urban & Fischer Verlag
Extent 571-578
Origin http://dx.doi.org/10.1016/j.etp.2016.09.003
Access rights Closed access
Type Article
Web of Science ID WOS:000389109200005
URI https://repositorio.unifesp.br/handle/11600/56809

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