Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

Author Calcagno, Danielle Queiroz Autor UNIFESP Google Scholar
Takeno, Sylvia Santomi Autor UNIFESP Google Scholar
Gigek, Carolina Oliveira Autor UNIFESP Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Wisnieski, Fernanda Autor UNIFESP Google Scholar
Chen, Elizabeth Suchi Autor UNIFESP Google Scholar
Araújo, Taíssa Maíra Thomaz Autor UNIFESP Google Scholar
Lima, Eleonidas Moura Autor UNIFESP Google Scholar
Melaragno, Maria Isabel Autor UNIFESP Google Scholar
Demachki, Samia Autor UNIFESP Google Scholar
Assumpção, Paulo Pimentel Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Abstract AIM To identify common copy number alterations on gastric cancer cell lines. METHODS Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha (IL11RA) and maternal embryonic leucine zipper kinase (MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11RA and MELK was observed in 19.1% (13/68) and 55.9% (38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.
Keywords IL11RA
Gastric cancer
Genomic profiling
MELK
9p13.3
xmlui.dri2xhtml.METS-1.0.item-coverage Pleasanton
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2009/07145-9
Date 2016
Published in World Journal Of Gastroenterology. Pleasanton, v. 22, n. 43, p. 9506-9514, 2016.
ISSN 1007-9327 (Sherpa/Romeo, impact factor)
Publisher Baishideng Publishing Group Inc
Extent 9506-9514
Origin http://dx.doi.org/10.3748/wjg.v22.i43.9506
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000389203400005
URI https://repositorio.unifesp.br/handle/11600/56706

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