Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine

Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine

Author Silva, Wemeson Ferreira da Autor UNIFESP Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Gutierre, Robson Campos Autor UNIFESP Google Scholar
Egami, Mizue Imoto Autor UNIFESP Google Scholar
Santos, Antenor Aguiar Autor UNIFESP Google Scholar
Antoniazzi, Marta M. Google Scholar
Sasso, Gisela Rodrigues da Silva Autor UNIFESP Google Scholar
Ranzani-Paiva, Maria Jose T. Google Scholar
Abstract Intestine mast cells/eosinophilic granule cells (MCs/EGC) of the marine species Centropomus parallelus (fat snook) were first studied using light and electron microscopy techniques. Mast cells are cells from the connective tissue found in almost all organs and tissues of vertebrates. In fish, they appear in greater numbers in parts of their bodies that are exposed to their environment, such as skin, gills and intestine. The granules in fat snook's mast cell contain a variety of substances, such as histamine, heparin, chondroitin sulfate, serotonin, proteases and cytokines. The present study of intestine MCs/EGC was carried out in 20 specimens of fat snook. Samples of tissue were fixed in Bouin solution and in buffered formalin. Ferric hematoxylin - Congo red, pH6 acridine orange, pH2.5 and pH0,5 Alcian Blue (AB), toluidine blue, PAS, AB + PAS and immunohistochemistry protocols were used. In the mucosa and submucosa layers, MCs/EGCs granules with basic contents were evidenced by Congo red staining, and with acid contents granules were identified through pH 2.5 and 0,5 AB, and acridine orange. Basic and acid contents were simultaneously evidenced using ferric hematoxylin - Congo red stain. Metachromasia was observed in both mucosal and submucosal mast cells. Neutral glycoproteins were evidenced by using PAS protocol, glycosaminoglycan through AB and both simultaneously through AB + PAS. In immunohistochemistry assays, MCs/EGC were positive for tryptase, chymase and serotonin. As in mammals, the study of samples fixed in modified Karnovsky for transmission electron microscopy evidenced that most of the MCs granules were spherical and showed varying electron density, as described in previous reports on other teleost fish species. The metachromasia observed and the identification of tryptase, chymase and serotonin suggest a great similarity between fat snook's MCs/EGC and those described in the mucosa of mammals. (C) 2016 Elsevier Ltd. All rights reserved.
Keywords Histochemistry
Immunohistochemistry
Ultrastructure
Mast cells
Centropomus parallelus
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor National Council for Scientific and Technological Development (CNPq) [559760/2009-4]
Coordination for the Improvement of Higher Education Personnel foundation (CAPES, CAPES PNPD) scholarship
Date 2017
Published in Fish & Shellfish Immunology. London, v. 60, p. 502-508, 2017.
ISSN 1050-4648 (Sherpa/Romeo, impact factor)
Publisher Academic Press Ltd- Elsevier Science Ltd
Extent 502-508
Origin http://dx.doi.org/10.1016/j.fsi.2016.11.022
Access rights Closed access
Type Article
Web of Science ID WOS:000392770000055
URI https://repositorio.unifesp.br/handle/11600/56472

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