Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice

Author Almeida-Reis, Rafael Google Scholar
Theodoro-Junior, Osmar A. Google Scholar
Oliveira, Bruno T. M. Google Scholar
Oliva, Leandro V. Google Scholar
Toledo-Arruda, Alessandra C. Google Scholar
Bonturi, Camila Ramalho Autor UNIFESP Google Scholar
Brito, Marlon Vilela de Autor UNIFESP Google Scholar
Lopes, Fernanda D. T. Q. S. Google Scholar
Prado, Carla Maximo Autor UNIFESP Google Scholar
Florencio, Ariana C. Google Scholar
Martins, Milton A. Google Scholar
Owen, Caroline A. Google Scholar
Leick, Edna A. Google Scholar
Oliva, Maria Luiza Vilela Autor UNIFESP Google Scholar
Tiberio, Iolanda F. L. C. Google Scholar
Abstract Background. Proteinases play a key role in emphysema. Bauhinia bauhinioides cruzipain inhibitor (BbCI) is a serine-cysteine proteinase inhibitor. We evaluated BbCI treatment in elastase-induced pulmonary alterations. Methods. C57BL/6 mice received intratracheal elastase (ELA group) or saline (SAL group). One group of mice was treated with BbCI (days 1, 15, and 21 after elastase instillation, ELABC group). Controls received saline and BbCI (SALBC group). After 28 days, we evaluated respiratory mechanics, exhaled nitric oxide, and bronchoalveolar lavage fluid. In lung tissue we measured airspace enlargement, quantified neutrophils, TNF alpha-, MMP-9-, MMP-12-, TIMP-1-, iNOS-, and eNOS-positive cells, 8-iso-PGF2 alpha, collagen,and elastic fibers in alveolar septa and airways. MUC-5-positive cells were quantified only in airways. Results. BbCI reduced elastase-induced changes in pulmonary mechanics, airspace enlargement and elastase-induced increases in total cells, and neutrophils in BALF. BbCI reduced macrophages and neutrophils positive cells in alveolar septa and neutrophils and TNF alpha-positive cells in airways. BbCI attenuated elastic and collagen fibers, MMP-9- and MMP-12-positive cells, and isoprostane and iNOS-positive cells in alveolar septa and airways. BbCI reduced MUC5ac-positive cells in airways. Conclusions. BbCI improved lung mechanics and reduced lung inflammation and airspace enlargement and increased oxidative stress levels induced by elastase. BbCI may have therapeutic potential in chronic obstructive pulmonary disease.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Faculdade de Medicina da Universidade de Sao Paulo, Brazil
Grant number Faculdade de Medicina da Universidade de Sao Paulo, Brazil: LIM-20-HC-FMUSP
Date 2017
Published in Biomed Research International. London, v. , p. -, 2017.
ISSN 2314-6133 (Sherpa/Romeo, impact factor)
Publisher Hindawi Ltd
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000399211200001

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