Astrocytic Expression of the Immunoreceptor CD300f Protects Hippocampal Neurons from Amyloid-beta Oligomer Toxicity In Vitro

Astrocytic Expression of the Immunoreceptor CD300f Protects Hippocampal Neurons from Amyloid-beta Oligomer Toxicity In Vitro

Author Lima, Thiago Zaqueu de Autor UNIFESP Google Scholar
Sardinha, Luis Roberto Google Scholar
Sayos, Joan Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Peluffo, Hugo Google Scholar
Abstract Background: Astrocytes contribute to neuroinflammation that accompanies neurodegenerative disorders such as Alzheimer's disease (AD). In this sense, the toxicity of these diseases might be attenuated through the modulation of astrocytic inflammatory responses. Recently, the CD300f immunoreceptor was described as a new member of the CD300 immunoreceptor family, showing promising modulatory properties. Objective: Here, we investigated whether overexpression of hCD300f (the human isoform of CD300f) in astrocytes protects hippocampal neurons against the degeneration induced by amyloid-beta (A beta) oligomer. Method: Astrocyte monolayers were transfected with hCD300f before seeding the hippocampal neurons, and then the co-culture was exposed to A beta(1-42) oligomers (5 mu M, 48h). Results: hCD300f expression significantly abrogated the neuronal loss elicited by A beta. This effect was dependent on neuron-astrocyte cell-cell interactions since no protection was observed using conditioned media from transfected astrocytes. Astrocyte modulation was dependent on the cytoplasmic signaling tail of hCD300f. Furthermore hCD300f expression did not affect the ability of astrocytes to uptake A beta(1-42) oligomers by endocytosis, which discards the possibility that increased A beta(1-42) clearance could mediate neuroprotection by hCD300f. Conclusion: These results suggest that the astrocyte-directed expression of the hCD300f immune receptor can be a neuroprotective strategy in AD disease.
Keywords Alzheimer's disease
amyloid-beta
Astrocytes
CD300f
neuroprotection
endocytosis
xmlui.dri2xhtml.METS-1.0.item-coverage Sharjah
Language English
Sponsor Fundacio Marato TV3, Catalunya, Spain
Comision Sectorial de Investigacion Cientifica (CSIC-UDELAR), Uruguay
Agencia Nacional de Investigacion e Innovacion (ANII), Uruguay
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)
Date 2017
Published in Current Alzheimer Research. Sharjah, v. 14, n. 7, p. 778-783, 2017.
ISSN 1567-2050 (Sherpa/Romeo, impact factor)
Publisher Bentham Science Publ Ltd
Extent 778-783
Origin http://dx.doi.org/10.2174/1567205014666170202121709
Access rights Closed access
Type Article
Web of Science ID WOS:000403945700009
URI https://repositorio.unifesp.br/handle/11600/56270

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