Leucine supplementation is anti-atrophic during paradoxical sleep deprivation in rats

Leucine supplementation is anti-atrophic during paradoxical sleep deprivation in rats

Author Souza, Helton de Sa Autor UNIFESP Google Scholar
Antunes, Hanna Karen Moreira Autor UNIFESP Google Scholar
Dattilo, Murilo Autor UNIFESP Google Scholar
Lee, Kil Sun Autor UNIFESP Google Scholar
Monico-Neto, Marcos Autor UNIFESP Google Scholar
Giampa, Sara Quaglia de Campos Autor UNIFESP Google Scholar
Phillips, Stuart M. Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Mello, Marco Tulio de Autor UNIFESP Google Scholar
Abstract The purpose of this study was to identify sleep deprivation-induced atrophy and the muscle-specific fiber types affected and to determine the effects of leucine supplementation on atrophy and pertinent portions of the pathways of muscle protein synthesis and degradation in rats. A total of 46 Wistar rats were distributed in four groups: control (CTL), leucine supplementation (LEU), sleep deprivation (SD), and leucine supplementation + sleep deprivation (LEU + SD). Leucine supplementation was by gavage (1.35 g/kg/daily), and the animals were subjected to SD for 96 h. Testosterone and corticosterone concentrations, along with proteins involved in protein synthesis and degradation and proteasome activity levels, were measured in the gastrocnemius (GA) muscle. Myosin ATPase staining was used to evaluate the different muscle fibers. After sleep deprivation, GA muscle and body masses decreased in the SD group compared to the CTL, LEU, and LEU + SD groups. There was no difference between groups in type I fiber cross-sectional area (CSA). The CSAs for type IIa fibers were lower in the SD and LEU + SD groups vs. the CTL and LEU groups, while the IIb fiber CSA was lower in the SD group vs. the CSAs in all other groups. The phospho (p)-Akt levels were lower in the SD and LEU + SD groups vs. the CTL and LEU groups. The p-mTORC1 levels were higher in the LEU, SD, and LEU + SD groups vs. the CTL group. The p-p70S6k levels were higher in the LEU and LEU + SD groups

the 4E-BP1 levels were higher in the SD and LEU + SD groups compared to those in the CTL and LEU groups, and the p-4E-BP1 levels were higher in the LEU and SD groups compared to those in the CTL group and even higher in the LEU + SD group compared to those in the LEU and SD groups. Ubiquitinated proteins, LC3, and p62/SQSTM, and proteasome activity levels were higher in the SD and LEU + SD groups vs. the LEU and CTL groups. Sleep deprivation led to the atrophy of IIa and IIb muscle fibers

however, leucine supplementation prevented muscle loss and type IIb fiber atrophy.
Keywords mTORC1
Skeletal muscle
Protein synthesis
Protein degradation
xmlui.dri2xhtml.METS-1.0.item-coverage Wien
Language English
Date 2016
Published in Amino Acids. Wien, v. 48, n. 4, p. 949-957, 2016.
ISSN 0939-4451 (Sherpa/Romeo, impact factor)
Publisher Springer Wien
Extent 949-957
Origin http://dx.doi.org/10.1007/s00726-015-2142-7
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372551100005
URI https://repositorio.unifesp.br/handle/11600/56161

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