Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells

Show simple item record

dc.contributor.author Nogueira, Alanderson R.
dc.contributor.author Leve, Fernanda
dc.contributor.author Morgado-Diaz, Jose
dc.contributor.author Tedesco, Roberto Carlos [UNIFESP]
dc.contributor.author Pereira, Mirian Claudia S.
dc.date.accessioned 2020-07-22T13:23:17Z
dc.date.available 2020-07-22T13:23:17Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.1017/S0031182015001973
dc.identifier.citation Parasitology. New York, v. 143, n. 5, p. 568-575, 2016.
dc.identifier.issn 0031-1820
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/56155
dc.description.abstract Ocular toxoplasmosis is the most frequent cause of uveitis, leading to partial or total loss of vision, with the retina the main affected structure. The cells of the retinal pigment epithelium (RPE) play an important role in the physiology of the retina and formation of the blood-retinal barrier. Several pathogens induce barrier dysfunction by altering tight junction (TJ) integrity. Here, we analysed the effect of infection by Toxoplasma gondii on TJ integrity in ARPE-19 cells. Loss of TJ integrity was demonstrated in T. gondii-infected ARPE-19 cells, causing increase in paracellular permeability and disturbance of the barrier function of the RPE. Confocal microscopy also revealed alteration in the TJ protein occludin induced by T. gondii infection. Disruption of junctional complex was also evidenced by scanning and transmission electron microscopy. Cell-cell contact loss was noticed in the early stages of infection by T. gondii with the visualization of small to moderate intercellular spaces. Large gaps were mostly observed with the progression of the infection. Thus, our data suggest that the alterations induced by T. gondii in the structural organization of the RPE may contribute to retinal injury evidenced by ocular toxoplasmosis. en
dc.description.sponsorship Fundacao Oswaldo Cruz/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (FIOCRUZ/CNPq)
dc.description.sponsorship Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorship Programa Estrategico de Apoio a Pesquisa em Saude (PAPES)
dc.format.extent 568-575
dc.language.iso eng
dc.publisher Cambridge Univ Press
dc.relation.ispartof Parasitology
dc.rights Acesso restrito
dc.subject Toxoplasma gondii en
dc.subject retinal pigment epithelial cells en
dc.subject junctional complex en
dc.title Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells en
dc.type Artigo
dc.description.affiliation Inst Oswaldo Cruz, Lab Ultraestrutura Celular, FIOCRUZ, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil
dc.description.affiliation Inst Oswaldo Cruz, Lab Biol Estrutural, FIOCRUZ, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil
dc.description.affiliation Inst Nacl Canc, Div Biol Celular, Coordenacao Pesquisa, Rua Andre Cavalcanti 37,5 Andar, BR-20230051 Rio De Janeiro, RJ, Brazil
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Disciplina Anat Descrit & Topog, Rua Botucatu 740, BR-04023900 Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Disciplina Anat Descrit & Topog, Rua Botucatu 740, BR-04023900 Sao Paulo, SP, Brazil
dc.description.sponsorshipID FIOCRUZ/CNPq: 7190637612315942
dc.description.sponsorshipID FAPERJ: E-26/010.001548/2014
dc.description.sponsorshipID PAPES: 4051089454296667
dc.identifier.doi 10.1017/S0031182015001973
dc.description.source Web of Science
dc.identifier.wos WOS:000373471200005
dc.coverage New York
dc.citation.volume 143
dc.citation.issue 5



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account