Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells

Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells

Author Nogueira, Alanderson R. Google Scholar
Leve, Fernanda Google Scholar
Morgado-Diaz, Jose Google Scholar
Tedesco, Roberto Carlos Autor UNIFESP Google Scholar
Pereira, Mirian Claudia S. Google Scholar
Abstract Ocular toxoplasmosis is the most frequent cause of uveitis, leading to partial or total loss of vision, with the retina the main affected structure. The cells of the retinal pigment epithelium (RPE) play an important role in the physiology of the retina and formation of the blood-retinal barrier. Several pathogens induce barrier dysfunction by altering tight junction (TJ) integrity. Here, we analysed the effect of infection by Toxoplasma gondii on TJ integrity in ARPE-19 cells. Loss of TJ integrity was demonstrated in T. gondii-infected ARPE-19 cells, causing increase in paracellular permeability and disturbance of the barrier function of the RPE. Confocal microscopy also revealed alteration in the TJ protein occludin induced by T. gondii infection. Disruption of junctional complex was also evidenced by scanning and transmission electron microscopy. Cell-cell contact loss was noticed in the early stages of infection by T. gondii with the visualization of small to moderate intercellular spaces. Large gaps were mostly observed with the progression of the infection. Thus, our data suggest that the alterations induced by T. gondii in the structural organization of the RPE may contribute to retinal injury evidenced by ocular toxoplasmosis.
Keywords Toxoplasma gondii
retinal pigment epithelial cells
junctional complex
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fundacao Oswaldo Cruz/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (FIOCRUZ/CNPq)
Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Programa Estrategico de Apoio a Pesquisa em Saude (PAPES)
Grant number FIOCRUZ/CNPq: 7190637612315942
FAPERJ: E-26/010.001548/2014
PAPES: 4051089454296667
Date 2016
Published in Parasitology. New York, v. 143, n. 5, p. 568-575, 2016.
ISSN 0031-1820 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 568-575
Origin http://dx.doi.org/10.1017/S0031182015001973
Access rights Closed access
Type Article
Web of Science ID WOS:000373471200005
URI https://repositorio.unifesp.br/handle/11600/56155

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