Serum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?

Serum anti-phenolic glycolipid1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?

Author Macedo, Alexandre C. de Google Scholar
Guimaraes, Juliana A. Google Scholar
Rodrigues, Raphael O. Google Scholar
Araujo, Thiago D. V. Google Scholar
Tavares, Clodis M. Google Scholar
Cabral, Paula B. Google Scholar
Moraes-Pinto, Maria Isabel de Autor UNIFESP Google Scholar
Nagao-Dias, Aparecida T. Google Scholar
Abstract ObjectiveThe aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. MethodAnalysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. ResultsA strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%

95% confidence interval) and 38.7% (24.4%-54.5%)

for IgG, 100% (87.0%-100.0%) and 28.8% (17.8%-42.1%), respectively

and for IgM, 95.2% (83.8%-99.4%) and 51.7% (32.5%-70.6%), respectively. ConclusionDespite the limiting factors, anti-PGL1 IgA correlates to IgM levels and it could be considered as a possible laboratorial tool to be also used, for instance, in serological follow-up studies.
Keywords IgA
IgG
IgM
isotypes
Mycobacterium leprae
native PGL1
serum antibodies
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor MCTI/CNPq/MS-SCTIE
Grant number MCTI/CNPq/MS-SCTIE: 403461/2012-0
Date 2018
Published in Journal Of Clinical Laboratory Analysis. Hoboken, v. 32, n. 3, p. -, 2018.
ISSN 0887-8013 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent -
Origin http://dx.doi.org/10.1002/jcla.22276
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000427567700001
URI https://repositorio.unifesp.br/handle/11600/55912

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