Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis

Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis

Autor Batista Júnior, João Google Scholar
Berzaghi, Rodrigo Autor UNIFESP Google Scholar
Arnaud, Adelita Duanra Medeiros do Monte Google Scholar
Fontes, Cor Jesus Fernandes Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Hahn, Rosane Christine Google Scholar
Instituição Universidade Federal de Mato Grosso Faculdade de Ciências Médicas Laboratório de Micologia
Universidade Federal de São Paulo (UNIFESP)
Universidade de Cuiabá Hospital Geral Universitário Laboratório de Micologia
Resumo This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
Palavra-chave Paracoccidioides brasiliensis
simultaneous infection
RAPD
Idioma Inglês
Data de publicação 2010-02-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 1, p. 62-65, 2010.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 62-65
Fonte http://dx.doi.org/10.1590/S0074-02762010000100009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02762010000100009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/5582

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