New myxosporeans parasitizing Phractocephalus hemioliopterus from Brazil: morphology, ultrastructure and SSU-rDNA sequencing

New myxosporeans parasitizing Phractocephalus hemioliopterus from Brazil: morphology, ultrastructure and SSU-rDNA sequencing

Author Naldoni, Juliana Autor UNIFESP Google Scholar
Maia, Antonio A. M. Google Scholar
Correa, Lincoln L. Google Scholar
Silva, Marcia R. M. da Google Scholar
Adriano, Edson Aparecido Autor UNIFESP Google Scholar
Abstract Myxozoans are a diverse group of parasitic cnidarians, with some species recognized as serious pathogens to their hosts. The present study describes 2 new myxobolid species (Myxobolus figueirae sp. nov. and Henneguya santarenensis sp. nov.) infecting skin and gill filaments of the Amazonian pimelodid fish Phractocephalus hemioliopterus, based on ultrastructural, histology and phylogenetic analysis. The fish were caught in the Amazon River, Para, Brazil. The plasmodial development of M. figueirae sp. nov. was in the dermis and those of H. santarenensis sp. nov. were of the intralamellar type. For both species, the plasmodia were surrounded by a connective tissue layer, but there was no inflammatory infiltrate. For M. figueirae sp. nov., mature spores were ovoid measuring 9.1 to 10 (9.5 +/- 0.3) mu m in length, 5.8 to 6.9 (6.4 +/- 0.3) mu m in width and 4.4 to 4.5 (4.5 +/- 0.1) mu m in thickness. Two polar capsules were elongated and of unequal size. For H. santarenensis sp. nov., mature spores were ellipsoidal in the frontal view, measuring 26.3 to 36.1 (31.9 +/- 3) mu m in total length, 9.6 to 11.9 (10.8 +/- 0.5) mu m in body length, 3.7 to 4.9 (4.3 +/- 0.3) mu m in width and 16.6 to 25.6 (21 +/- 3.1) mu m in caudal process. The polar capsules were elongated and of equal size. Phylogenetic analysis, based on partial small subunit ribosomal DNA (SSU rDNA) sequences and using the closest myxozoan sequences to each one of the species studied here based on previous GenBank data, showed M. figueirae sp. nov. and H. santarenensis sp. nov. clustering in distinct lineages. While H. santarenensis sp. nov. clustered in a well-supported subclade composed of Henneguya species that infect gills of South American pimelodid hosts, M. figueirae sp. nov. clustered in a weakly supported subclade containing parasite species of bryconid hosts.
Keywords Myxosporea
Myxobolus figueirae
Henneguya santarenensis
Ultrastructure
Histology
Amazon
Brazil
xmlui.dri2xhtml.METS-1.0.item-coverage Oldendorf Luhe
Language English
Sponsor Sao Paulo Research Foundation - FAPESP
Brazilian Fostering Agency CNPq
Grant number FAPESP: 2013/21374-6
FAPESP: 2014/22700-7
CNPq: 305630/2013-0
Date 2018
Published in Diseases Of Aquatic Organisms. Oldendorf Luhe, v. 128, n. 1, p. 37-49, 2018.
ISSN 0177-5103 (Sherpa/Romeo, impact factor)
Publisher Inter-Research
Extent 37-49
Origin http://dx.doi.org/10.3354/dao03210
Access rights Closed access
Type Article
Web of Science ID WOS:000428737800004
URI https://repositorio.unifesp.br/handle/11600/55804

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