Physico-chemical characterization and biocompatibility of hydroxyapatite derived from fish waste

Physico-chemical characterization and biocompatibility of hydroxyapatite derived from fish waste

Author Yamamura, Hirochi Autor UNIFESP Google Scholar
Silva, Victor Hugo Pereira da Autor UNIFESP Google Scholar
Ruiz, Pedro Luiz Menin Google Scholar
Ussui, Valter Autor UNIFESP Google Scholar
Lazar, Dolores Ribeiro Ricci Google Scholar
Renno, Ana Claudia Muniz Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Abstract The aim of this study was to synthesize hydroxyapatite (HAP) powder from fish waste. The powder was characterized through X-ray diffraction, Fourier transform infrared spectroscopy, ion exchange chromatography, scanning electron microscopy and plasma emission spectrometry. The cyto- and genotoxicity was carried out to demonstrate biocompatibility in vivo by means of rat subcutaneous tissue test. The results showed that the visible crystalline nature of typical apatite crystal structure when they were calcined at 800 degrees C. Infrared spectroscopy analysis showed similar composition to HAP standard with the presence of carbonate ion demonstrated by wave number values of 871 cm(-1) and 1420 cm(-1) for calcinations at 800 degrees C. The scanning electronmicrographies depicted the crystal morphology and porous nature with average pore size of similar to 10 mu m. Plasma emission spectrometry and ion exchange chromatography confirmed the presence of Ca and P in the samples. The mean of calcium content was 36.8

Mg was 0.8, Na was 0.7 and K was 0.5. Rat subcutaneous tissue test revealed that HAP presented biocompatibility. Furthermore, the lack of cyto- and genotoxicity in blood, liver, kidney and lung were noticed after 30 days of HAP implantation. Taken together, our results demonstrated that HAP from fish waste exhibits a great potential for using as biomaterial since is represents a simple, effective, low-cost process and satisfactory degree of biocompatibility.
Keywords Hydroxyapatite
Biomaterials
Biocompatibility
Rat
Whitemouth croaker
Fish waste
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
Grant number CNPq: 442149/2014-0
Date 2018
Published in Journal Of The Mechanical Behavior Of Biomedical Materials. Amsterdam, v. 80, p. 137-142, 2018.
ISSN 1751-6161 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 137-142
Origin http://dx.doi.org/10.1016/j.jmbbm.2018.01.035
Access rights Closed access
Type Article
Web of Science ID WOS:000428486800018
URI https://repositorio.unifesp.br/handle/11600/55764

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