Astaxanthin Restrains Nitrative-Oxidative Peroxidation in Mitochondrial-Mimetic Liposomes: A Pre-Apoptosis Model

Astaxanthin Restrains Nitrative-Oxidative Peroxidation in Mitochondrial-Mimetic Liposomes: A Pre-Apoptosis Model

Author Mano, Camila M. Google Scholar
Guaratini, Thais Google Scholar
Cardozo, Karina H. M. Google Scholar
Colepicolo, Pio Google Scholar
Bechara, Etelvino José Henriques Autor UNIFESP Google Scholar
Barros, Marcelo P. Google Scholar
Abstract Astaxanthin (ASTA) is a ketocarotenoid found in many marine organisms and that affords many benefits to human health. ASTA is particularly effective against radical-mediated lipid peroxidation, and recent findings hypothesize a "mitochondrial-targeted" action of ASTA in cells. Therefore, we examined the protective effects of ASTA against lipid peroxidation in zwitterionic phosphatidylcholine liposomes (PCLs) and anionic phosphatidylcholine: phosphatidylglycerol liposomes (PCPGLs), at different pHs (6.2 to 8.0), which were challenged by oxidizing/nitrating conditions that mimic the regular and preapoptotic redox environment of active mitochondria. Pre-apoptotic conditions were created by oxidized/nitr(osyl) ated cytochrome c and resulted in the highest levels of lipoperoxidation in both PCL and PCPGLs (pH 7.4). ASTA was less protective at acidic conditions, especially in anionic PCPGLs. Our data demonstrated the ability of ASTA to hamper oxidative and nitrative events that lead to cytochrome c-peroxidase apoptosis and lipid peroxidation, although its efficiency changes with pH and lipid composition of membranes.
Keywords mitochondria
lipid peroxidation
oxidative stress
xmlui.dri2xhtml.METS-1.0.item-coverage Basel
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP
BPE fellowship)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (Bolsa Produtividade em Pesquisa, Nivel 2, CNPq, Brazil)
Programa Iberoamericano de Ciencia y Tecnologia para el Desarollo (CYTED
Red iberoamericana para el estudio de nuevos carotenoides bioactivos como ingredientes de alimentos, Spain)
Grant number FAPESP: 017/06032-2
CNPq: 304663/2015-8
RIENCBI: 112RT0445
Date 2018
Published in Marine Drugs. Basel, v. 16, n. 4, 2018.
ISSN 1660-3397 (Sherpa/Romeo, impact factor)
Publisher Mdpi
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000431068000028

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