Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes

Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes

Author Grabulosa, Caren Cristina Autor UNIFESP Google Scholar
Manfredi, Silvia Regina Autor UNIFESP Google Scholar
Canziani, Maria Eugênia Fernandes Autor UNIFESP Google Scholar
Quinto, Beata Marie Redublo Autor UNIFESP Google Scholar
Barbosa, Rodrigo B. Google Scholar
Rebello, Jacqueline F. Google Scholar
Batista, Marcelo Costa Autor UNIFESP Google Scholar
Cendoroglo, Miguel Autor UNIFESP Google Scholar
Dalboni, Maria Aparecida Autor UNIFESP Google Scholar
Abstract TLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 expression in neutrophils and monocytes from HD and CKD patients. Blood samples were drawn from 47 CKD and 43 HD patients and 71 age and gender-matched healthy volunteers (CONT). TLR4 was analyzed using flow cytometry. Cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 were analyzed via ELISA.TLR4 expression in neutrophils was higher in HD patients than in stage 3 and 4 CKD patients. In these cells, we observed a positive correlation between TLR4 and cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 levels. In monocytes, TLR4 expression was significantly higher in CKD 3 and 4 groups than in the control and HD groups and positively and negatively correlated with IL-6 and MCP-1 and cathelicidin, respectively. TNF-alpha, IL-6 and MCP-1 serum levels were higher in HD and CKD patients than in control. Cathelicidin and IL-10 levels were only higher in HD patients. IL-6 serum levels were positively correlated with all cytokines, and cathelicidin was negatively correlated with MCP-1 (r = - 0.35

p < 0.01) and positively correlated with IL-10 (r = 0.37

p = 0.001). These results suggest that a uremic environment induces high TLR4, cathelicidin and cytokine expression and may increase inflammation. Thus, future studies should be conducted to evaluate whether TLR4 and cathelicidin should be targets for anti-inflammatory therapeutic strategies.
Keywords Toll like receptors
Toll like receptors
Inflammation
Inflammation
Chronic kidney disease
Chronic kidney disease
Immunology
Immunology
Cathelicidin
Cathelicidin
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Sponsor FAPESP
Grant number FAPESP: 2010/52180-0
FAPESP: 2011/51496-0
Date 2018
Published in Experimental Cell Research. San Diego, v. 365, n. 2, p. 157-162, 2018.
ISSN 0014-4827 (Sherpa/Romeo, impact factor)
Publisher Elsevier Inc
Extent 157-162
Origin http://dx.doi.org/10.1016/j.yexcr.2018.02.022
Access rights Closed access
Type Article
Web of Science ID WOS:000429630000001
URI https://repositorio.unifesp.br/handle/11600/55634

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