Retinal Toxicity of Acai Fruit (Euterpe Oleracea) Dye Concentrations in Rabbits: Basic Principles of a New Dye for Chromovitrectomy in Humans

Retinal Toxicity of Acai Fruit (Euterpe Oleracea) Dye Concentrations in Rabbits: Basic Principles of a New Dye for Chromovitrectomy in Humans

Author Caiado, Rafael R. Autor UNIFESP Google Scholar
Peris, Cristiane S. Autor UNIFESP Google Scholar
Souza Lima-Filho, Acacio Alves Autor UNIFESP Google Scholar
Palma Urushima, Joao Guilherme Autor UNIFESP Google Scholar
Novais, Eduardo Autor UNIFESP Google Scholar
Badaro, Emmerson Autor UNIFESP Google Scholar
Maia, Andre Autor UNIFESP Google Scholar
Sinigaglia-Coimbra, Rita Autor UNIFESP Google Scholar
Watanabe, Sung Eun S. Google Scholar
Rodrigues, Eduardo B. Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Maia, Mauricio Autor UNIFESP Google Scholar
Abstract Purpose: Evaluate toxicity of acai fruit (Euterpe oleracea) dye concentrations in a rabbit model.Methods: Rabbits were injected intravitreously with 10%, 25%, and 35% acai dye concentrations. Control eyes received balanced salt solution (BSS). Electroretinogram (ERG), fundus imaging, fluorescein angiography (FA), optical coherence tomography (OCT), and light and transmission electron microscopy (LM/TEM) were performed.Results: Fundus imaging showed increased vitreous opacity with increased dye concentrations. FA and OCT showed normality with all concentrations. Comparisons between BSS and dye concentrations were analyzed using Kruskal-Wallis and Mood's median test (p < 0.05). At 24 h, ERGs showed reduced amplitudes from baseline in all eyes. Median b-wave amplitudes nonsignificantly decreased and latency increased with 10% and 25%

findings were significant (p < 0.05) for 35%. LM and TEM showed no abnormalities for 10% and 25%. With 35%, TEM showed ganglion cell edema at 24 h that resolved after 7 days. Vacuolization, multilamellar bodies, and nerve bundle damage occurred at 24 h/7 days in the inner nuclear layer. Mitochondrial cristae disruption occurred in the inner photoreceptor segment at 24 h that decreased by 7 days.Conclusion: Ten and twenty-five percent concentrations were safe and may improve identification of the posterior hyaloid and internal limiting membrane during chromovitrectomy in humans.
Keywords Acai fruit
anthocyanins
chromovitrectomy
Euterpe oleracea
xmlui.dri2xhtml.METS-1.0.item-coverage Philadelphia
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil)
Pan-American Association of Ophthalmology/Pan-American Ophthalmological Foundation, Paul Kayser Global Award (PAAO/PAOF, Arlington, Texas).
Date 2017
Published in Current Eye Research. Philadelphia, v. 42, n. 8, p. 1185-1193, 2017.
ISSN 0271-3683 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 1185-1193
Origin http://dx.doi.org/10.1080/02713683.2017.1297995
Access rights Closed access
Type Article
Web of Science ID WOS:000407080800017
URI https://repositorio.unifesp.br/handle/11600/55494

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