Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal

Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal

Author Devoz, Paula Picoli Google Scholar
Gomes, Willian Robert Google Scholar
De Araujo, Marilia Ladeira Autor UNIFESP Google Scholar
Ribeiro, Diego Luis Google Scholar
Pedron, Tatiana Google Scholar
Greggi Antunes, Lusania Maria Google Scholar
Batista, Bruno Lemos Google Scholar
Barbosa, Fernando, Jr. Google Scholar
Mazzaron Barcelos, Gustavo Rafael Autor UNIFESP Google Scholar
Abstract Previous studies showed that lead (Pb) exposure may modulate gene expression by changes in the epigenetic status. However, little is known about the impact of Pb exposure and alterations on DNA methylation patterns in humans exposed to this metal. The aim of this study was to assess the consequences of exposure to Pb on DNA global methylation, in order to gain a better understanding of the interactions between Pb exposure and epigenetic effects. The study included 100 male workers employed in automotive battery factories from Parana State, Brazil. Concentrations of Pb in blood (B-Pb) and plasma (P-Pb) were determined by ICP-MS, the percentage (%) of global DNA methylation was determined by quantification of 5-methylcytosine using indirect ELISA, and sociodemographic data collected by questionnaire by trained interviewers. The mean age was 37 +/- 10 (18-67 years)

18% of participants were smokers, while 32% reported consumption of alcoholic beverages. The B-Pb and P-Pb levels were 20 +/- 11 and 0.56 +/- 0.64 mu g/ dl, respectively

% global DNA methylation was 2.8 +/- 1.1% (ranging from 1.1 to 6.5%). B-Pb and P-Pb concentrations were significantly correlated. Furthermore, a marked association was noted between Pb biomarkers and DNA global methylation. Taken together, our data demonstrated that Pb exposure induced alterations on DNA global methylation in workers who were exposed to the metal and consequently may result in disturbances in the regulation of gene expression, leading to potentially several health adverse effect outcomes.
xmlui.dri2xhtml.METS-1.0.item-coverage Philadelphia
Language English
Sponsor Sao Paulo Research Foundation (FAPESP)
Coordination for the Improvement of Higher Education Personnel (CAPES)
Grant number FAPESP: 2013/06033-8
FAPESP: 2014/24981-3
FAPESP: 2015/04786-4
FAPESP: 2015/07277-3
Date 2017
Published in Journal Of Toxicology And Environmental Health-Part A-Current Issues. Philadelphia, v. 80, n. 19-21, p. 1098-1105, 2017.
ISSN 1528-7394 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent 1098-1105
Origin http://dx.doi.org/10.1080/15287394.2017.1357364
Access rights Closed access
Type Article
Web of Science ID WOS:000416348400006
URI https://repositorio.unifesp.br/handle/11600/55427

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