Nasal Polyp-Derived Mesenchymal Stromal Cells Exhibit Lack of Immune-Associated Molecules and High Levels of Stem/Progenitor Cells Markers

Nasal Polyp-Derived Mesenchymal Stromal Cells Exhibit Lack of Immune-Associated Molecules and High Levels of Stem/Progenitor Cells Markers

Author Barbosa de Oliveira, Pedro Wey Autor UNIFESP Google Scholar
Pezato, Rogerio Autor UNIFESP Google Scholar
Henao Agudelo, Juan Sebastian Autor UNIFESP Google Scholar
Perez-Novo, Claudina Angela Google Scholar
Berghe, Wim Vanden Google Scholar
Camara, Niels Olsen Autor UNIFESP Google Scholar
de Almeida, Danilo Candido Google Scholar
Gregorio, Luis Carlos Autor UNIFESP Google Scholar
Abstract Mesenchymal stromal cells (MSCs) are considered adult progenitor stem cells and have been studied in a multitude of tissues. In this context, the microenvironment of nasal polyp tissue has several inflammatory cells, but their stroma compartment remains little elucidated. Hence, we isolated MSCs from nasal polyps Polyp-MSCs (PO-MSCs) and compared their molecular features and gene expression pattern with bone marrow-derived MSCs (BM-MSCs). Initially, both PO-MSCs and BM-MSCs were isolated, cultivated, and submitted to morphologic, differentiation, phenotypic, immunosuppressive, and gene expression assays. Compared to BM-MSCs, PO-MSCs showed normal morphology and similar osteogenic/ adipogenic differentiation potential, but their immunophenotyping showed lack of immune-associated molecules (e. g., CD117, HLA-DR, PDL-1, and PDL2), which was linked with less immunoregulatory abilities such as (i) inhibition of lymphocytes proliferation and (ii) regulatory T cell expansion. Furthermore, we detected in the PO-MSCs a distinct gene expression profile in comparison with BM-MSCs. PO-MSC expressed higher levels of progenitor stem cells specific markers (e. g., CD133 and ABCB1), while BM-MSCs showed elevated expression of cytokines and growth factors (e. g., FGF10, KDR, and GDF6). The gene ontology analysis showed that the differentially modulated genes in PO-MSC were related with matrix remodeling process and hexose and glucose transport. For BM-MSCs, the highly expressed genes were associated with behavior, angiogenesis, blood vessel morphogenesis, cell-cell signaling, and regulation of response to external stimulus. Thus, these results suggest that PO-MSCs, while sharing similar aspects with BM-MSCs, express a different profile of molecules, which presumably can be implicated in the development of nasal polyp tissue.
Keywords mesenchymal stromal cells
nasal polyposis
gene expression
immunoregulation
stem cells
xmlui.dri2xhtml.METS-1.0.item-coverage Lausanne
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Grant number FAPESP: 12/02270-2
Date 2017
Published in Frontiers In Immunology. Lausanne, v. 8, p. -, 2017.
ISSN 1664-3224 (Sherpa/Romeo, impact factor)
Publisher Frontiers Media Sa
Extent -
Origin http://dx.doi.org/10.3389/fimmu.2017.00039
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000392746500001
URI https://repositorio.unifesp.br/handle/11600/55229

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