Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

Author Siqueira das Neves, Lais Mara Google Scholar
Marcondes Ferreira Leite, Gabriella de Paula Google Scholar
Marcolino, Alexandre Marcio Google Scholar
Pinfildi, Carlos Eduardo Autor UNIFESP Google Scholar
Garcia, Sergio Britto Google Scholar
de Araujo, Joao Eduardo Google Scholar
de Oliveira Guirro, Elaine Caldeira Google Scholar
Abstract The aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flap

group 2 (laser 830 nm) underwent the surgical technique and was irradiated with a laser 830 nm

group 3 (laser 660 nm) underwent the surgical technique and was irradiated with a laser 660 nm

group 4 was treated with nicotine subcutaneously (2 mg/kg/2x/day/4 weeks) and underwent surgery

group 5 (nicotine + laser 830 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 830 nm

group 6 (nicotine + laser 660 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 660 nm. The application of PBM occurred immediately after surgery and on the two following days. The percentage of necrosis was assessed using the AxioVisionA (R) software. The number of mast cells (toluidine blue staining) was evaluated, and immunohistochemistry was performed to detect vascular endothelial growth factor expression (anti-VEGF-A), fibroblasts (anti-basic FGF), and neoformed vessels (anti-CD34). PBM with a wavelength of 830 nm increased the viability of the TRAM flap, with a smaller area of necrosis, increased number of mast cells, and higher expression of VEGF and CD34. PBM increases the viability of musculocutaneous flaps treated with to nicotine.
Keywords Low-level laser therapy
Phototherapy
Dosimetry
Physical therapy
Vascular endothelial growth factor
Fibroblast growth factor
Nicotine
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Foundation of Sao Paulo State Research (FAPESP)
Grant number FAPESP: 2013/07602-6
FAPESP: 2013/07732-7
Date 2017
Published in Lasers In Medical Science. London, v. 32, n. 2, p. 335-341, 2017.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer London Ltd
Extent 335-341
Origin http://dx.doi.org/10.1007/s10103-016-2118-1
Access rights Closed access
Type Article
Web of Science ID WOS:000394154600010
URI https://repositorio.unifesp.br/handle/11600/55189

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