Evaluating the presence of Toxoplasma gondii in peripheral blood of patients with diverse forms of uveitis

Evaluating the presence of Toxoplasma gondii in peripheral blood of patients with diverse forms of uveitis

Author Belfort, Rubens N. Autor UNIFESP Google Scholar
Isenberg, Jordan Google Scholar
Fernandes, Bruno F. Google Scholar
Di Cesare, Sebastian Google Scholar
Belfort, Rubens, Jr. Autor UNIFESP Google Scholar
Burnier, Miguel N., Jr. Autor UNIFESP Google Scholar
Abstract The purpose of this study was to evaluate the presence of Toxoplasmosis gondii in samples of peripheral blood from patients with varying etiologies of uveitis. Whole blood from patients with different forms of uveitis was tested for the presence of T. gondii using real-time PCR targeting the well-characterized 529 bp fragment. Extracted DNA was both frozen. Thirty-one patients were included in the current study and grouped as follows: acute toxoplasmosis (n = 10)

toxoplasmic retinal scars (n = 9)

non-infectious etiologies of uveitis (n = 6)

and IgG negative for toxoplasmosis (n = 6). In total, only two patients were shown to have circulating T. gondii in peripheral blood

both of these patients were IgG positive for toxoplasmosis, were receiving immunosuppressive therapy for autoimmune uveitis, and had no clinical features of toxoplasmosis. T. gondii was identified in peripheral blood of some immunosuppressed patients. No other patients, including those with acute toxoplasmosis, had circulating parasites in peripheral blood.
Keywords PCR
Toxoplasma gondii
Uveitis
xmlui.dri2xhtml.METS-1.0.item-coverage Dordrecht
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil)
Date 2017
Published in International Ophthalmology. Dordrecht, v. 37, n. 1, p. 19-23, 2017.
ISSN 0165-5701 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 19-23
Origin http://dx.doi.org/10.1007/s10792-016-0221-8
Access rights Closed access
Type Article
Web of Science ID WOS:000394159800004
URI https://repositorio.unifesp.br/handle/11600/55187

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