Medial Ganglionic Eminence Cells Freshly Obtained or Expanded as Neurospheres Show Distinct Cellular and Molecular Properties in Reducing Epileptic Seizures

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dc.contributor.author Romariz, Simone A. A. [UNIFESP]
dc.contributor.author Paiva, Daisylea S. [UNIFESP]
dc.contributor.author Galindo, Layla T. [UNIFESP]
dc.contributor.author Barnabe, Gabriela F.
dc.contributor.author Guedes, Vivian A.
dc.contributor.author Borlongan, Cesario V.
dc.contributor.author Longo, Beatriz M. [UNIFESP]
dc.date.accessioned 2020-07-17T14:03:08Z
dc.date.available 2020-07-17T14:03:08Z
dc.date.issued 2017
dc.identifier http://dx.doi.org/10.1111/cns.12650
dc.identifier.citation Cns Neuroscience & Therapeutics. Hoboken, v. 23, n. 2, p. 127-134, 2017.
dc.identifier.issn 1755-5930
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/55186
dc.description.abstract AimsMedial ganglionic eminence (MGE) progenitors give rise to inhibitory interneurons and may serve as an alternative cell source for large-scale cell transplantation for epilepsy after in vitro expansion. We investigated whether modifications in the culture medium of MGE neurospheres affect neuronal differentiation and expression of MGE-specific genes. In vivo, we compared anticonvulsant effects and cell differentiation pattern among neurospheres grown in different culture media and compared them with freshly harvested MGE cells. MethodsWe used four variations of cell culture: standard, containing growth factors (EGF/FGF-2) (GF) en
dc.description.abstract addition of retinoic acid (GF-RA) en
dc.description.abstract withdrawal of EGF/FGF-2 (WD) en
dc.description.abstract and addition of retinoic acid and withdrawal of EGF/FGF-2 (WD-RA). Based on in vitro results neurosphere-grown (WD-RA or GF conditions) or fresh MGE cells were transplanted into the hippocampus. ResultsIn vitro WD-RA showed increased neuronal population and higher expression of Dlx1, Nkx2.1, and Lhx6 genes in comparison with GF culture condition. After transplantation, fresh MGE cells and neurospheres (GF) showed anticonvulsant effects. However, fresh MGE cells differentiated preferentially into inhibitory neurons, while GF gave rise to glial cells. ConclusionWe conclude that freshly isolated and neurosphere-grown MGE cells reduced seizures by different mechanisms (inhibitory interneurons vs. astrocytes). Fresh MGE cells appear more appropriate for cell therapies targeting inhibitory interneurons for conferring anticonvulsant outcomes. en
dc.description.sponsorship FAPESP
dc.description.sponsorship CNPq
dc.format.extent 127-134
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Cns Neuroscience & Therapeutics
dc.rights Acesso restrito
dc.subject Epilepsy en
dc.subject Interneuronal progenitors en
dc.subject Medial ganglionic eminence en
dc.subject Neurospheres en
dc.subject Transplantation en
dc.title Medial Ganglionic Eminence Cells Freshly Obtained or Expanded as Neurospheres Show Distinct Cellular and Molecular Properties in Reducing Epileptic Seizures en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Dept Fisiol, Sao Paulo, SP, Brazil
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliation Ludwig Inst Canc Res, Inst Sirio Libanes Ensino & Pesquisa, Sao Paulo, SP, Brazil
dc.description.affiliation Univ S Florida, Dept Neurosurg & Brain Repair, Tampa, FL USA
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Dept Fisiol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Dept Bioquim, Sao Paulo, SP, Brazil
dc.identifier.doi 10.1111/cns.12650
dc.description.source Web of Science
dc.identifier.wos WOS:000394157900003
dc.coverage Hoboken
dc.citation.volume 23
dc.citation.issue 2



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