Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo

Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo

Autor Machado, Alexandre Paulo Autor UNIFESP Google Scholar
Anzai, Mariana Caselli Google Scholar
Gompertz, Olga Fischman Autor UNIFESP Google Scholar
Instituição Federal University of Mato Grosso Department of Basic Health Sciences
Universidade Federal de São Paulo (UNIFESP)
Resumo Interactions among microorganisms may be the cause of morphological modifications, particularly in fungal cells. The aim of this work was to examine the changes that occur in cells of the fungus Fonsecaea pedrosoi after in vitro co-culturing with Bacillus subtilis and to explore the results of this interaction in vivo in an experimental murine infection. B. subtilis strain was inoculated into a 15-day pure culture of F. pedrosoi. In vitro, after 48 hours of co-culturing, the fungal cells were roundish. The secretion of fungal dark pigments and production of terminal chlamydoconidia were observed in hyphae after one week. In the in vivo study, two animal groups of 30 BALB/c mice each were employed. One group was inoculated intraperitoneally with hyphal fragments from the co-culture of bacteria and fungi; the other group was infected only with F. pedrosoi hyphae. After seven days of infection, both animal groups developed neutrophilic abscesses. Phagocytosis of bacilli by macrophages occurred at three days. At later periods, generally after 25 days, only roundish cells similar to sclerotic bodies remained in the tissues while hyphae were eliminated by 15 to 20 days. These fungal forms originated mainly from terminal chlamydoconidia. The co-culturing between bacteria and fungi may constitute a mechanism to rapidly obtain resistant fungal forms for host defenses, especially for chromoblastomycosis (CBM) experimental infections.
Palavra-chave Bacillus subtilis
Fonsecaea pedrosoi
microbial interaction
co-culture
fungal antagonism
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
FAPEMAT
Número do financiamento FAPEMAT: 002.138/2007
Data de publicação 2010-01-01
Publicado em Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 16, n. 4, p. 592-598, 2010.
ISSN 1678-9199 (Sherpa/Romeo, fator de impacto)
Publicador Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Extensão 592-598
Fonte http://dx.doi.org/10.1590/S1678-91992010000400009
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000284778000009
SciELO S1678-91992010000400009 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/5513

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