Immunohistochemical assessment of cell populations in leprosy-spectrum lesions and reactional forms

Immunohistochemical assessment of cell populations in leprosy-spectrum lesions and reactional forms

Author Vincenzi Fachin, Luciana Raquel Google Scholar
Soares, Cleverson Teixeira Google Scholar
Fernandes Belone, Andrea de Faria Google Scholar
Favaro Trombone, Ana Paula Google Scholar
Rosa, Patricia Sammarco Google Scholar
Guidella, Cassio Cesar Google Scholar
Franco, Marcello Fabiano Autor UNIFESP Google Scholar
Abstract In situ immunophenotyping of leprosy lesions can improve our understanding of the biology of inflammatory cells during the immune response to Mycobacterium leprae antigens. In the present study, biopsies from 10 healthy controls and 70 leprosy patients were selected, 10 for each of the following conditions: clinical tuberculoid (TT), borderline tuberculoid (BT), borderline borderline (BB), borderline lepromatous (BL), lepromatous (LL), reversal reaction (R1), and erythema nodosum leprosum (R2). Qualitative and quantitative immunohistochemical analyses were performed to detect CD3, CD4, CD8, FoxP3, CD20, CD138, CD1a, CD57, CD15, CD117, CD68, and CD163. In addition, histochemistry was employed to identify eosinophils. The amount of CD3(+) and CD4(+) T cells was higher in TT than in LL patients. CD8(+) T cells were predominant in T lymphocyte infiltrations in the basal layer of the epidermis. The number of FoxP3(+) cells was similar among different forms of the disease, but was higher in BL and LL than in R2 individuals. CD20(+) lymphocytes were most abundant in TT samples, while CD138(+) plasma cells displayed no detectable differences. Epithelioid macrophages from the center of TT and R1 granulomas exhibited the M1 phenotype (CD68(+) CD163(-)), whereas those in LL granulomas showed the M2 phenotype (CD68(+) CD163(+)). There was a gradual decrease in the amount of CD1a(+) cells from the TT towards the LL form of the disease. A significant increase in the number of neutrophils was observed only in R2 samples. All the cells investigated, except eosinophils, participated in the immunopathogenesis of leprosy.
Keywords Leprosy
M. leprae
Pathogenesis
Cellular infiltrate
Immunopathology
xmlui.dri2xhtml.METS-1.0.item-coverage Murcia
Language English
Sponsor Fundacao Paulista Contra a Hanseniase, Sao Paulo, Brasil
Date 2017
Published in Histology And Histopathology. Murcia, v. 32, n. 4, p. 385-396, 2017.
ISSN 0213-3911 (Sherpa/Romeo, impact factor)
Publisher F Hernandez
Extent 385-396
Origin http://dx.doi.org/10.14670/HH-11-804
Access rights Closed access
Type Article
Web of Science ID WOS:000392387100008
URI https://repositorio.unifesp.br/handle/11600/54904

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