BMP8B Is a Tumor Suppressor Gene Regulated by Histone Acetylation in Gastric Cancer

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dc.contributor.author Wisnieski, Fernanda [UNIFESP]
dc.contributor.author Leal, Mariana Ferreira [UNIFESP]
dc.contributor.author Calcagno, Danielle Queiroz
dc.contributor.author Santos, Leonardo Caires [UNIFESP]
dc.contributor.author Gigek, Carolina Oliveira [UNIFESP]
dc.contributor.author Chen, Elizabeth Suchi [UNIFESP]
dc.contributor.author Artigiani, Ricardo [UNIFESP]
dc.contributor.author Demachki, Samia
dc.contributor.author Assumpcao, Paulo Pimentel
dc.contributor.author Lourenco, Laercio Gomes [UNIFESP]
dc.contributor.author Burbano, Rommel Rodriguez
dc.contributor.author Smith, Marilia Cardoso [UNIFESP]
dc.date.accessioned 2020-07-17T14:02:34Z
dc.date.available 2020-07-17T14:02:34Z
dc.date.issued 2017
dc.identifier http://dx.doi.org/10.1002/jcb.25766
dc.identifier.citation Journal Of Cellular Biochemistry. Hoboken, v. 118, n. 4, p. 869-877, 2017.
dc.identifier.issn 0730-2312
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/54869
dc.description.abstract Different from genetic alterations, the reversible nature of epigenetic modifications provides an interesting opportunity for the development of clinically relevant therapeutics in different tumors. In this study, we aimed to screen and validate candidate genes regulated by the epigenetic marker associated with transcriptional activation, histone acetylation, in gastric cancer ( GC). We first compared gene expression profile of trichostatin A-treated and control GC cell lines using microarray assay. Among the 55 differentially expressed genes identified in this analysis, we chose the up-regulated genes BMP8B and BAMBI for further analyses, that included mRNA and histone acetylation quantification in paired GC and nontumor tissue samples. BMP8B expression was reduced in GC compared to nontumor samples (P < 0.01). In addition, reduced BMP8B expression was associated with poorly differentiated GC ( P = 0.02). No differences or histopathological associations were identified concerning BAMBI expression. Furthermore, acetylated H3K9 and H4K16 levels at BMP8B were increased in GC compared to nontumors ( P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC ( P < 0.05). Reduced levels of acetylated H3K9 was also associated with diffuse-type histological GC ( P < 0.05). Notably, reduced BMP8B mRNA and acetylated H4K16 levels were positively correlated in poorly differentiated GC ( P < 0.05). Our study demonstrated that BMP8B seems to be a tumor suppressor gene regulated by H4K16 acetylation in poorly differentiated GC. Therefore, BMP8B may be a potential target for TSA-based therapies in this GC sample subset. (C) 2016 Wiley Periodicals, Inc. en
dc.description.sponsorship Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorship Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.format.extent 869-877
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof Journal Of Cellular Biochemistry
dc.rights Acesso restrito
dc.subject GENE EXPRESSION REGULATION en
dc.subject HISTONE ACETYLATION en
dc.subject TRICHOSTATIN A en
dc.subject GASTRIC CANCER en
dc.subject BMP8B en
dc.subject BAMBI en
dc.title BMP8B Is a Tumor Suppressor Gene Regulated by Histone Acetylation in Gastric Cancer en
dc.type Artigo
dc.description.affiliation Univ Fed Sao Paulo, Disciplina Genet, Dept Morfol & Genet, Rua Botucatu 740, BR-04023900 Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Dept Ortopedia & Traumatol, Rua Borges Lagoa 783, BR-04038032 Sao Paulo, Brazil
dc.description.affiliation |Fed Univ Para, Hosp Joao de Barros Barreto, Nucleo Pesquisas Oncol, Ave Mundurucus 4487, BR-66073000 Belem, Para, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Disciplina Gastroenterol Cirurg, Dept Cirurgia, Rua Napoleo de Barros 715, BR-04024002 Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Dept Patol, Rua Botucatu 740, BR-04023000 Sao Paulo, Brazil
dc.description.affiliation Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, Rua Augusto Correia 01, BR-66075110 Belem, Para, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Disciplina Genet, Dept Morfol & Genet, Rua Botucatu 740, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Disciplina Gastroenterol Cirurg, Dept Cirurgia, Rua Napoleo de Barros 715, BR-04024002 Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Patol, Rua Botucatu 740, BR-04023000 Sao Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2009/07145-9
dc.description.sponsorshipID CAPES: 201567/2009-1
dc.identifier.doi 10.1002/jcb.25766
dc.description.source Web of Science
dc.identifier.wos WOS:000397420300022
dc.coverage Hoboken
dc.citation.volume 118
dc.citation.issue 4



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