High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates

High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates

Author Passos-Castilho, Ana Maria Autor UNIFESP Google Scholar
Hernandes Granato, Celso Francisco Autor UNIFESP Google Scholar
Abstract Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Parana State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus RNA was detected in 34 (20.0%) of the 170 pigs following positive results in at least one set of screening real-time RT-PCR primers and probes. The swine hepatitis E virus strains clustered with the genotype hepatitis E virus-3b reference sequences in the phylogenetic analysis and showed close similarity to human hepatitis E virus isolates previously reported in Brazil. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Keywords Brazil
Genotype 3
Hepatitis E virus
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2012/22925-3, 2013.03701-0]
Date 2017
Published in Brazilian Journal Of Microbiology. Sao Paulo, v. 48, n. 2, p. 373-379, 2017.
ISSN 1517-8382 (Sherpa/Romeo, impact factor)
Publisher Soc Brasileira Microbiologia
Extent 373-379
Origin http://dx.doi.org/10.1016/j.bjm.2016.10.022
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000401456800029
URI https://repositorio.unifesp.br/handle/11600/54791

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